Efficient Protocol for Improving the Development of Cryopreserved Embryonic Axes of Chestnut ( Mill.) by Encapsulation-Vitrification.

An optimized cryopreservation protocol for embryonic axes (EAs) of chestnut ( Mill.) has been developed based on the encapsulation-vitrification procedure. EAs of mature seeds were aseptically dissected and encapsulated in alginate beads with or without 0.