Publications by authors named "Tamara V Tsulaia"
Background: Scanning cytometry now has many of the features (and power) of multiparameter flow cytometry while keeping its own advantages as an imaging technology. Modern instruments combine capabilities of scanning cytometry with the ability to manipulate cells. A new technology, called LEAP (laser-enabled analysis and processing), offers a unique combination of capabilities in cell purification and selective macromolecule delivery (optoinjection).
View Article and Find Full Text PDFArticle Synopsis
- Human mesenchymal stem cells (hMSCs) can differentiate into various tissue types, making them promising for cell and gene therapies.
- The study demonstrated that glass needle-mediated microinjection effectively delivered macromolecules like DNA to hMSCs, achieving a 76% cell viability post-injection.
- Microinjection of supercoiled DNA vectors resulted in significantly higher gene expression and the formation of long-lasting GFP-positive colonies, indicating potential for future genetic modifications in hMSCs.