Cryopreservation at -20 and -70 °C of Pleurotus ostreatus on Grains.

Alternative substrates for cryopreservation at -20 °C have been little explored for basidiomycetes and could bring new possibilities of lower cost cryopreservation. Nevertheless, freezing temperatures between -15 and -60 °C are very challenging because they frequently result in cryoinjuries. The objective of this study was to evaluate substrates associated to cryoprotective agents for Pleurotus ostreatus cryopreservation at -20 or -70 °C in order to develop alternative techniques for basidiomycete cryopreservation.