Synthesis of poly(N-isopropylacrylamide-co-2-hydroxyethyl methacrylate) with low polydispersity using ultrasonic irradiation.

Poly(N-isopropylacrylamide-co-2-hydroxyethyl methacrylate) having low polydispersity was synthesized in mixed solvent of ethanol and water using ultrasonic irradiation without any chemical polymerization initiator. The effects of the volume fraction of ethanol in the solvent, the molar ratio of two monomers, the monomer concentration and the ultrasonic power intensity on the time courses of the conversion to the polymer, the number average molecular weight, and the polydispersity of synthesized polymer were investigated in order to determine the optimal conditions to synthesize the copolymers with a narrow molecular weight distribution (i.e.

Determination of serum lipoprotein lipase using a latex particle-enhanced turbidimetric immunoassay with an automated analyzer.

Background: Lipoprotein lipase (LPL) plays a central role in triglyceride-rich lipoprotein metabolism by catalyzing the hydrolysis of triglycerides. Quantification of serum LPL is useful for diagnosing lipid disorders, but there is no rapid method of measuring LPL for clinical use.

Methods: We developed a rapid and sensitive latex particle-enhanced turbidimetric immunoassay (LTIA) serum LPL using latex bead-immobilized anti-LPL monoclonal antibodies.