Expanded roles of lactate-sensing LldR in transcription regulation of the K-12 genome: lactate utilisation and acid resistance.

LldR is a lactate-responsive transcription factor (TF) that transcriptionally regulates the operon consisting of lactate permease and lactate dehydrogenase. The operon facilitates the utilisation of lactic acid in bacteria. However, the role of LldR in whole genomic transcriptional regulation, and the mechanism involved in adaptation to lactate remains unclear.

Expanded roles of pyruvate-sensing PdhR in transcription regulation of the K-12 genome: fatty acid catabolism and cell motility.

The transcription factor PdhR has been recognized as the master regulator of the pyruvate catabolism pathway in , including both NAD-linked oxidative decarboxylation of pyruvate to acetyl-CoA by PDHc (pyruvate dehydrogenase complex) and respiratory electron transport of NADH to oxygen by Ndh-CyoABCD enzymes. To identify the whole set of regulatory targets under the control of pyruvate-sensing PdhR, we performed genomic SELEX (gSELEX) screening . A total of 35 PdhR-binding sites were identified along the K-12 genome, including previously identified targets.

Author Correction: Regulatory Role of PlaR (YiaJ) for Plant Utilization in Escherichia coli K-12.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Regulatory Role of PlaR (YiaJ) for Plant Utilization in Escherichia coli K-12.

Outside a warm-blooded animal host, the enterobacterium Escherichia coli K-12 is also able to grow and survive in stressful nature. The major organic substance in nature is plant, but the genetic system of E. coli how to utilize plant-derived materials as nutrients is poorly understood.