Publications by authors named "Taketo Obitsu"

This study aimed to clarify the efficacy of cashew nutshell liquid (CNSL) in methane emissions, milk production, and rumen fermentation of lactating cows in practical conditions. Ten Holstein lactating cows were used in a free-stall barn with a milking robot. Two treatments were arranged as control (no CNSL additive, n = 5) or CNSL addition (10 g/day of CNSL, n = 5) for 21 days after the 7-day preliminary period.

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  • This research aims to improve the quality of rice bran (RB) and de-oiled rice bran (DORB) by using fermentation with rumen microbes from sheep, leading to beneficial chemical changes.
  • The fermentation process involved various moisture levels and durations, resulting in a decrease in harmful compounds like phytate-P and crude fiber, while increasing valuable nutrients like inorganic phosphorus.
  • The study concludes that fermenting RB for 12 hours and DORB for up to 72 hours can make these byproducts more suitable as feed for non-ruminant animals like poultry and pigs, especially with moisture levels up to 50%.
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  • Sake lees, a byproduct of Japanese rice wine, are high in protein and ethanol and were studied as feed for sheep, focusing on their effects on rumen fermentation and digestion.
  • The study used a 4 × 4 Latin square design with four sheep, testing ensiled versus non-ensiled total mixed rations (TMR) with different types of sake lees.
  • Results showed that while dietary ethanol was higher for those on ensiled TMR, it negatively affected dry matter and nitrogen digestion, with lower plasma amino acid concentrations, indicating potential concerns when formulating such feeds.
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Extracts of Acacia and Quebracho have been used as a feed additive in ruminant diets; the effects, however, have been varied. This study used a meta-analysis approach to evaluate the use of those extracts on nutrient utilization, performance, and methane production of ruminants. A database was developed from 37 published papers comprising 152 dietary treatments.

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Background And Aim: As a non-protein nitrogen source, urea is a popular, low cost, and easily obtained protein supplement. The objective of the present study was to perform a meta-analysis of the effects of urea supplementation on rumen fermentation and sheep performance.

Materials And Methods: A total of 32 experiments from 21 articles were compiled into a dataset.

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Chitosan (CHI) has been used as a feed additive in ruminant diets, but the effects obtained to date have been varied. This study aimed to evaluate the dietary addition of CHI on performance, nutrient utilization, and product characteristics of ruminants by using a meta-analysis approach. A total of 15 articles that composed of 21 studies and 57 data points were included in the database.

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The aim of this study was to develop prediction equations for methane (CH ) emissions from lactating cows using the CH /carbon dioxide (CO ) ratio in the breath measured in the automatic milking system (AMS) and to evaluate the predicted values and factors affecting the CH /CO ratio. The model development was conducted using a dataset determined in respiration chambers or head boxes (n = 121). Then, gas measurements in the AMS as well as in the head box were carried out with six lactating cows fed one of three different levels of neutral detergent fiber (NDF) content, following a 3 × 3 Latin square experimental design.

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Farm managers' decision to cull dairy cows is based on the cows' milk production, history of disorder(s), and reproductive performance, each of which affects dairy cows' lifetime (herd life and productive lifespan). We investigated the relationships among the incidence of clinical mastitis (CM), the reproductive performance, and the culling rate. We also assessed the effects of these relationships on the lifetimes of dairy cows, using the records made before and after the introduction of an automatic milking system (AMS) at Hiroshima University Farm.

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Objective: Bioactive compounds in ruminant products are related to functional compounds in their diets. Therefore, this study aimed to explore the effect of forage sources, Italian ryegrass (IR) silage vs corn silage (CS) in the total mixed ration (TMR), on milk production, milk composition, and phytanic acid content in milk, as well as on the extent of conversion of dietary phytol to milk phytanic acid.

Methods: Phytanic acid content in milk was investigated for cows fed a TMR containing either IR silage or CS using 17 cows over three periods of 21 days each.

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  • The study assessed how ensiling (fermenting) fruit byproducts like persimmon peel and grape pomace impacts their chemical makeup and fermentation in sheep's stomachs (rumen).
  • It found that ensiling reduced non-fiber carbohydrates, especially in persimmon peel, but total tannins remained stable up to 4 weeks, with some soluble tannins decreasing.
  • The ensiled byproducts generally led to lower gas and fatty acid production, but adding polyethylene glycol (PEG) helped increase gas production, indicating that the effects of ensiling on fermentation may vary between different fruit byproducts.
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The objective of this experiment was to test the effects of combining plant extracts rich in tannins and saponins at varying proportions on in vitro ruminal methane and ammonia formation. Tannins were extracted from leaves and saponins from fruits with various solvents. The extracts obtained with the most efficient solvents (tannins: 75% water and 25% methanol; saponins: pure methanol) were then used in vitro.

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The effects of inclusion of persimmon peel (PP) in total mixed ration (TMR) silage on its nutrient composition, tannin content, and in vitro ruminal fermentation were studied. Four types of TMR silages containing 0, 50, 100, and 150 g/kg of PP on a dry matter basis were prepared. The dietary contents of non-fiber carbohydrate (NFC) decreased, while soluble protein fraction increased after ensiling of the TMR.

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The effect of blue LED on melatonin secretion, feeding behaviour and growth was addressed in Holstein female dairy calves. In Exp.1, six animals (8 weeks old, 97 ± 4.

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This study aimed to investigate the effect of an ensiling period (Experiment 1) and adding lactic acid bacteria (LAB, Experiment 2) on the changes in carotenoid, chlorophyll, and phytol in ensiled Italian ryegrass (IR, Lolium multiflorum Lam.). In Experiment 1, the IR herbage ensiled into plastic bags was analyzed for the contents of photosynthetic pigments and phytol over a 5-week period.

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  • Three rumen-protected lysine (RPL) products were tested for stability in two different forage-based total mixed rations (TMR), focusing on their lysine release over time.
  • In the experiments, the RPL products showed varying levels of lysine release percentage based on the diet's dry matter (DM) content and the duration of exposure, with the third product (C) performing the best.
  • The study also found a correlation between the lysine release in water and the diets, suggesting that Brix value could be a useful indicator for assessing the effectiveness of RPL protection.
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Single amino acids (AA) feeding is gaining more attention for their functional roles beyond nutritional needs. This study aimed to describe the endocrine and metabolic responses to a single AA administration (at 10% of MP for maintenance) in 48 hr fasted sheep (n = 4) receiving, over continued 4 hr, a duodenal infusate of saline (control), glutamate (Glu), glutamine (Gln), lysine HCl (Lys), threonine (Thr), or valine (Val) in a 4 by 6 Youden square design with weekly intervals. Blood samples were collected at 0, 15, 30, 60, 120, 180, and 240 min relative to the infusion onset, and plasma AA, glucose, β-hydroxybutyric acid (BHBA), nonesterified fatty acids (NEFA), ghrelin, insulin, glucagon, and insulin-like growth factor 1 (IGF-1) concentrations were measured.

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This study aimed to investigate the effects of replacing alfalfa hay (AH) with a mixture of cassava foliage silage and sweet potato vine silage (CSP) (1:1 on a dry matter (DM) basis) on ruminal and intestinal nutrient digestion in sheep. Four wethers were fed a control diet containing 35% of AH and two treatment diets containing 15% and 30% of the CSP as substitute for AH at 1.5 times the metabolizable energy required for maintenance.

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This study aimed to investigate the effects of nitrogen (N) fertilizer application and harvesting stage on the contents of chlorophyll, phytol and carotenoids (β-carotene and lutein) in Italian ryegrass herbage before and after ensiling, and the extent of phytol preservation after ensiling. Three rates of N fertilizer (0, 60 and 120 kg N/ha) were applied by top-dressing as an additional fertilizer. The herbage harvested at booting stage (27 weeks of age) or heading stage (29 weeks of age) were wilted for 1 day, then ensiled for 60 days using a small-scale pouch system.

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The present study aimed to determine the effects of differing nutrient levels during the far-off period on postpartum metabolism and milk production in lactating cows. Twenty-six multiparous cows were assigned to three dietary treatments in the far-off period: a low-energy diet (L, n = 9, 80% intake of the total digestible nutrients requirement), a moderate-energy diet (M, n = 8, 105%) and a high-energy diet (H, n = 9, 130%). During the close-up period, all cows were provided with 105% intake.

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This study was conducted to identify the insulin-independent actions of glucagon-like peptide-1 (GLP-1 (7-36 amide)) in partitioning nutrient metabolism in ovine liver. Four Suffolk wethers (60.0 ± 6.

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The objective of this study was to investigate the effects of the hot summer season on plasma glucose and oxidative stress markers. For two 14-day experimental periods, namely periods 1 (July-August) and 2 (October-November), 12 and 14 lactating dairy cows, respectively, that were milked using an automatic milking system, were fed diets containing similar ingredients, and their milk production, plasma metabolites and oxidative status markers were investigated. Dry matter intake and milk yield were not affected by the experimental period.

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The objective of this study was to clarify the effect of different milking frequencies under an automatic milking system (AMS) on milk yield, plasma metabolite profiles and mammary arterial-venous (A-V) differences of milk precursors by mammary tissues in early lactation cows. Twelve Holstein cows were divided into two and four times milking frequency treatments by AMS after calving to 50 days postpartum. Cows were given a partial mixed ration ad libitum and a concentrate diet at every milking.

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Insulin-independent actions of glucagon-like peptide-1 (GLP-1) are not yet clear in ruminants. Four Suffolk mature wethers (60.0 ± 6.

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Nitrogen (N) digestion and urea-N metabolism in Hokkaido native horses fed roughage-based diets containing different types and levels of protein sources were studied. Horses (173 ± 4.8 kg) fitted with an ileum cannula were fed four diets consisting of 100% timothy hay (TH), 88% TH and 12% soybean meal (SBM), 79% TH and 21% SBM, and 51% TH and 49% alfalfa hay at 2.

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Four Holstein steers were used to evaluate the combination effects of whole crop corn (Cs) or rice (Rs) silage with steam-flaked corn (Cg) or rice (Rg) grain (four dietary treatments) on ruminal carbohydrate digestion, duodenal nitrogen (N) flow and plasma essential amino acid (EAA) concentration. The ruminal digestibility of starch and nonfiber carbohydrate (NFC) for Rs and Rg diets compared with Cs and Cg diets was greater, but that of neutral detergent fiber (aNDFom) was less. Because the ruminal disappearance of NFC plus aNDFom was similar across four dietary treatments, microbial N flow was not affected by the diets.

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