Publications by authors named "Takeshi Yabana"
Purpose: To investigate sectoral differences in the relationship between intraocular pressure (IOP) dynamics during dark-room prone testing (DRPT) and visual field (VF) defect progression in primary open-angle glaucoma (POAG) patients.
Design: Retrospective, longitudinal study.
Participants: This retrospective study included 116 eyes of 84 POAG patients who underwent DRPT and had at least 5 reliable VF tests conducted over a more than 2-year follow-up period.
Introduction: Abnormal findings on optical coherence tomography (OCT) and electroretinography (ERG) have been reported in participants with schizophrenia spectrum disorders (SSDs). This study aims to reveal the pooled standard mean difference (SMD) in retinal parameters on OCT and ERG among participants with SSDs and healthy controls and their association with demographic characteristics, clinical symptoms, smoking, diabetes mellitus, and hypertension.
Methods: Using PubMed, Scopus, Web of Science, and PSYNDEX, we searched the literature from inception to March 31, 2023, using specific search terms.
Article Synopsis
- - The study aimed to explore how changes in intraocular pressure (IOP) during Dark-Room Prone Testing (DRPT) relate to IOP levels over a two-year period in patients with primary open-angle glaucoma.
- - A total of 84 eyes from 51 patients were analyzed, focusing on IOP measurements taken both in a sitting position and after lying prone in a dark room, with a focus on IOP fluctuations and maximum values.
- - Results showed a significant increase in IOP after DRPT, suggesting that short-term IOP responses can help predict long-term IOP changes in glaucoma patients.
Article Synopsis
- The study examined retinal changes in patients with schizophrenia spectrum disorders (SSDs) to understand their correlation with clinical factors.
- A systematic review included 23 studies, revealing that SSD patients had significantly thinner retinal layers and lower macular volume compared to healthy controls.
- Findings suggest that these retinal abnormalities could serve as potential markers for the condition, but further longitudinal research is needed to confirm these results.
Purpose: To investigate the effect of plant-derived antioxidant compounds, identified with primary culture screening, on retinal ganglion cell (RGC) survival in mice under excitotoxic conditions. Additionally, to determine the effect of these compounds on the involvement of calpain inactivation.
Materials And Methods: Plant-derived antioxidant compounds including hesperidin, crocetin, and were administrated orally to C57BL/6J mice.
Glaucoma is a leading cause of blindness worldwide and is characterized by degeneration associated with the death of retinal ganglion cells (RGCs). It is believed that glaucoma is a group of heterogeneous diseases with multifactorial pathomechanisms. Here, we investigate whether anti-inflammation treatment with an ER stress blockade can selectively promote neuroprotection against NMDA injury in the RGCs.
View Article and Find Full Text PDFThe genetically encoded green fluorescent protein-based calcium sensor, GCaMP, has been used to detect calcium transients and report neuronal activity. We evaluated the specificity of GCaMP3 expression to retinal ganglion cells (RGCs) of the transgenic Thy1-GCaMP3 mouse line in healthy control animals and in those after optic nerve transection (ONT). Retinas from control mice (n = 4) were isolated and stained for RNA-binding protein with multiple splicing (RBPMS) and choline acetyltransferase (ChAT), specific markers for RGCs and cholinergic amacrine cells, respectively.
View Article and Find Full Text PDFPurpose: To investigate the effect of mitochondrial dysfunction on the autoregulation of blood flow, by measuring levels of glutathione, an indicator of mitochondrial dysfunction, in glaucoma patients.
Methods: Fifty-six OAG patients and 21 age-matched controls underwent a blood assay. Mitochondrial function was measured according to the levels of total glutathione (t-GSH), reduced GSH (GSH), and oxidized GSH (GSSG, glutathione disulfide) in peripheral blood mononuclear cells.
Purpose: GCaMP3 is a genetically encoded calcium indicator for monitoring intracellular calcium dynamics. We characterized the expression pattern and functional properties of GCaMP3 in the Thy1-GCaMP3 transgenic mouse retina.
Methods: To determine the specificity of GCaMP3 expression, Thy1-GCaMP3 (B6; CBA-Tg(Thy1-GCaMP3)6Gfng/J) retinas were processed for immunohistochemistry with anti-green fluorescent protein (anti-GFP, to enhance GCaMP3 fluorescence), anti-RBPMS (retinal ganglion cell [RGC]-specific marker), and antibodies against amacrine cell markers (ChAT, GABA, GAD67, syntaxin).
Purpose: To assess the therapeutic potential of endothelin-converting enzyme-like 1 (Ecel1) in a mouse model of optic nerve crush.
Methods: Ecel1 expression was evaluated with real time quantitative (qRT)-PCR, Western blotting, and immunohistochemistry in mouse retinas after optic nerve crush. Vinblastine administration to the optic nerve and the intravitreal injection of N-methyl-d-aspartate (NMDA) were used to assess Ecel1 gene expression.
Article Synopsis
- Hesperidin, a plant-based bioflavonoid, shows potential as a neuroprotective treatment for retinal damage after testing 41 materials for antioxidant properties.
- Intravitreal injections of hesperidin in mice prevented retinal ganglion cell death and reduced harmful factors like oxidative stress and inflammation following NMDA-induced excitotoxicity.
- The treatment also improved overall retinal function, suggesting hesperidin could be a therapeutic supplement for conditions like glaucoma and diabetic retinopathy.
Purpose: To investigate a novel optical coherence tomography (OCT)-derived variable, circumpapillary mean retinal shadow width (cpMRSW), and to elucidate its association with normal-tension glaucoma (NTG).
Methods: For the purpose of validation, we measured retinal vascular calibers in 68 arterioles and 100 venules of 12 NTG patients and 12 healthy subjects and compared the width of the visible retinal shadows in spectral-domain OCT images and the caliber of retinal vessels in retinal photographs. Then we calculated cpMRSW in 78 NTG eyes and 25 age-matched healthy control eyes.