C-metabolic flux analysis reveals metabolic rewiring in HL-60 neutrophil-like cells through differentiation and immune stimulation.

Neutrophils are innate immune cells and the first line of defense for the maintenance of homeostasis. However, our knowledge of the metabolic rewiring associated with their differentiation and immune stimulation is limited. Here, quantitative C-metabolic flux analysis was performed using HL-60 cells as the neutrophil model.

mfapy: An open-source Python package for C-based metabolic flux analysis.

C-based metabolic flux analysis (C-MFA) is an essential tool for estimating intracellular metabolic flux levels in metabolic engineering and biology. In C-MFA, a metabolic flux distribution that explains the observed isotope labeling data was computationally estimated using a non-linear optimization method. Herein, we report the development of mfapy, an open-source Python package developed for more flexibility and extensibility for C-MFA.