Publications by authors named "Takeo Sugiyama"
Article Synopsis
- The study investigated the role of phosphodiesterase 4 (PDE4) in HMG human malignant melanoma cells, finding that PDE4 activity is present and can be inhibited by rolipram.
- Specific PDE4 inhibitors and forskolin were shown to increase intracellular cAMP levels, which then influenced cell growth.
- The research indicates that targeting PDE4 could potentially be a new therapeutic strategy for treating human malignant melanoma, as previous studies have not explored this positive regulation of cell growth through PDE4 inhibition.
Phosphodiesterase (PDE) 3 has been characterized in isolated rat submandibular acini. PDE3 activity was detected in homogenates of isolated rat submandibular acini; little or no PDE3 activity was found in ducts. About 62% of PDE3 activity in the acini was recovered in the supernatant fractions; 38% in particulate fractions.
View Article and Find Full Text PDFReverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that HOSM-1 cells, an osteosarcoma cell line established from human mandible, expressed mRNA for osteoblastic markers, such as alkaline phosphatase, osteonectin, osteocalcin and parathyroid hormone receptor, thus exhibiting an osteoblastic phenotype. We have investigated a possible role of cyclic nucleotide phosphodiesterases (PDEs) in osteosarcoma cells. RT-PCR analysis revealed that HOSM-1 cells expressed mRNA for PDE4A, 4B and 4C.
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