Cytoprotective Effect of Pteryxin on Insulinoma MIN6 Cells Due to Antioxidant Enzymes Expression via Nrf2/ARE Activation.

The low-level antioxidant activity of pancreatic islets causes type 1 diabetes due to oxidative stress, which is also the cause of failure in the pancreatic islets' isolation and cell transplantation. In our previous study, pteryxin was found to be a natural product as a nuclear factor-erythroid-2-related factor (Nrf2) activator. This study focused on elucidation that the potentiality of pteryxin can activate the antioxidant enzymes, even under oxidative stress, by hydrogen peroxide (HO).

Development of energy intensive multifunction cavitation technology and its application to the surface modification of the Ni-based columnar crystal superalloy CM186LC.

The present work demonstrates a technique for the hot forging of metal surfaces in water at 1000 °C or higher, termed energy-intensive multifunctional cavitation (EI-MFC). In this process, the energy of cavitation bubbles is maximized, following which these bubbles collide with the metal surface. This technique will be employed to improve the surface structure of CM186LC/DS, a Ni-based columnar crystalline superalloy used to manufacture the rotor blades of jet engines and gas turbines that are exposed to high-temperature oxidizing environments, with the aim of improving creep strength.

Purified Gymnemic Acids from Tea Inhibit 3T3-L1 Cell Differentiation into Adipocytes.

(GI) is an indigenous medicinal plant and functional food in Thailand that has recently helped to reduce plasma glucose levels in healthy humans. It is renowned for the medicinal properties of gymnemic acid and its ability to suppress glucose absorption. However, the effects of gymnemic acids on adipogenesis that contribute to the accumulation of adipose tissues associated with obesity remain unknown.

High-temperature corrosion behavior of high-temperature and high-pressure cavitation processed Cr-Mo steel surface.

In this paper, long-term high-temperature corrosion at 500 °C and high-temperature corrosion at the melting temperature of a corrosive ash mixture were examined because the use of high-temperature equipment such as boilers and gas turbines increases year over year. To investigate the optimum cavitation processing conditions for the specimens used in high-temperature corrosion tests, the surface properties of each processed specimen were examined. In specimens processed using multifunction cavitation (MFC), the compressive residual stress was high when the processing time was 10 min and the Cr content on the surface was greater than on the surface of an unprocessed specimen.

Nitric Oxide Modulation by Folic Acid Fortification.

Folic acid (FA) can be protected the neural tube defects (NTDs) causing nitric oxide (NO) induction, but the alleviation mechanism of the detailed FA function against NO has not yet been clarified. This study focused on elucidation of the interaction of FA and NO. FA suppressed nitrite accumulation as the NO indicator in lipopolysaccharide (LPS)-stimulated RAW264.

Induction of Antioxidant Protein HO-1 Through Nrf2-ARE Signaling Due to Pteryxin in Thunb in RAW264.7 Macrophage Cells.

This study focused on exploring the nuclear factor-erythroid-2-related factor (Nrf2) active compound to avoid oxidative stress related to various diseases, such as obesity and diabetes mellitus. The activity of the Nrf2-ARE (antioxidant response element) signaling was evaluated by a reporter assay involving over five hundred various edible medicinal herbs, and the highest Nrf2 activity was found in the ethanol extract of leaves. The active compound in the extract was isolated by high performance liquid chromatography (HPLC), and the chemical structure was identical to pteryxin based on H, C-NMR spectra and liquid chromatography/time-of-fright mass spectrometer (LC/TOF/MS).

Antioxidant capacity of betacyanins as radical scavengers for peroxyl radical and nitric oxide.

This study was designed to assess the antioxidant capacity of betacyanins as indole derived plant pigments, such as betanin, phyllocactin and betanidin. The antioxidant capacity of the betacyanins was evaluated as an index of radical scavenging ability using the peroxyl radical generating system in the presence of AAPH and NO generating system using NOR3 as an NO donor. The peroxyl radical scavenging capacity was dose-dependent in the low concentration range (25-100 nM).

Suppression of nitric oxide production on LPS/IFN-γ-stimulated RAW264.7 macrophages by a novel catechin, pilosanol N, from Agrimonia pilosa Ledeb.

A novel catechin, pilosanol N (1), was isolated from Agrimonia pilosa Ledeb and its structure was determined by (1)H, (13)C NMR and HRESI-MS analyses. Compound 1 inhibited nitric oxide (NO) production in LPS/IFN-γ -induced RAW264.7 macrophages, and then the iNOS gene expression and its protein production with LPS/IFN-γ treatment cells were also suppressed in the presence of 1.

Isolation of C₁₁ compounds and a cyclopropane fatty acid from an Okinawan ascidian, Diplosoma sp.

Pentylphenols 1 and 2, cyclopropane fatty acid 3, and cyclopentenones 4 and 5, were isolated from an ascidian, Diplosoma sp. The structures of 1-5 were determined by spectroscopic analysis and/or synthesis. Compound 1 inhibited the division of fertilized sea urchin eggs and compound 4 showed mild cytotoxity against HCT116 cells (human colorectal cancer cell).

Melanin synthesis inhibitors from Balanophora fungosa.

Tyrosinase, trypsin, and tryptase are known to play important roles in melanin production of human skin. This paper describes the study of the inhibitory effect of Balanophora fungosa on melanin. The 50% EtOH extract obtained from B.

Isolation of C11 cyclopentenones from two didemnid species, Lissoclinum sp. and Diplosoma sp.

A series of new C(11) cyclopentenones 1-7 was isolated, together with four known metabolites 9/10, 12 and 13, from the extract of the didemnid ascidian Lissoclinum sp. The other didemnid ascidian Diplosoma sp. contained didemnenones 1, 2 and 5, and five known metabolites 8-12.

Cytotoxic metabolites from the Okinawan ascidian Diplosoma virens.

The unstable isomeric compounds 5-hydroxy-7-prop-2-en-(E)-ylidene-7,7adihydro-2H-cyclopenta[b]pyran-6-one (1) and 5-hydroxy-7-prop-2-en-(Z)-ylidene-7,7adihydro-2H-cyclopenta[b]pyran-6-one (2), previously described as antimicrobial metabolites from the sponge Ulosa sp., were isolated and identified as major components of the ascidian Diplosoma virens. In this paper, full spectral data for 2 and complete 13CNMR data for 1, based on 2D NMR measurements, are provided for the first time.