Publications by authors named "Takatomo Fujiyama"
A rapid and reliable method for determining irradiation histories of ground beef and prawns was developed on the basis of a method for determining the irradiation history of beef liver by liquid chromatography-tandem mass spectrometry (LC-MS/MS) of 5,6-dihydrothymidine (DHdThd). Improvements in the method included the following: (1) 50% ethanol precipitation in the DNA extraction step was conducted before the RNase step, (2) snake venom phosphodiesterase I was used for DNA digestion to boost liberation of DHdThd, and (3) a matrix-matched calibration curve was used for determining DHdThd by LC-MS/MS analysis. This method successfully determined irradiation histories of ground beef and prawns.
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- - A method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed to detect irradiation histories in raw beef livers by measuring the compound 5,6-dihydrothymidine (DHdThd).
- - The process involved extracting liver DNA, digesting it enzymatically, and purifying nucleosides, followed by analysis where DHdThd levels increased in a dose-dependent manner after γ-irradiation between 1.0-11.3 kGy.
- - This technique shows promise for identifying irradiation history in food products where DNA can be extracted, particularly noting consistent dose-response results across different beef livers.