Melting temperature mapping method in children: Rapid identification of pathogenic microbes.

Introduction: The melting temperature (T) mapping method (TM) identifies bacterial species by intrinsic patterns of T values in the 16S ribosomal RNA gene (16S rDNA) extracted directly from whole blood. We examined potential clinical application of TM in children with bloodstream infection (BSI).

Methods: This was a prospective observational study at a children's hospital in Japan from 2018 to 2021.