Projection range of eDNA analysis in marshes: a suggestion from the Siberian salamander () inhabiting the Kushiro marsh, Japan.

Background: Freshwater ecosystems are rapidly declining. The Siberian salamander () which inhabits the Kushiro marsh in Hokkaido, Japan has lost some habitat due to human activity. There are many challenges associated with conventional monitoring methods, including cost, the need for specialist personnel, environmental impact, and ability to detect the presence of this species; thus, we investigated the feasibility of using environmental DNA (eDNA) analysis to detect its presence and identify its breeding grounds.