Publications by authors named "Takao Karasawa"
Background: In addition to regulating the antiviral response, increased expression of Toll-like receptor 3 (TLR3) in resident renal cells plays a role in developing some forms of glomerulonephritis. TLR3 activation leads to type I interferon (IFN) production, which induces the expression of IFN-stimulated genes (ISGs). However, the role of ISG20 expression in resident renal cells remains unclear.
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- Dysregulated immune responses during viral infections can worsen the disease and lead to poor outcomes, highlighting the importance of pathways like JAK/STAT in immune function.
- Tryptanthrin, a natural compound, shows potential therapeutic effects, particularly by reducing the activation of a key immune signaling protein, p-STAT1, in macrophages when stimulated by the TLR3 pathway.
- The study indicates that tryptanthrin specifically inhibits the immune response triggered by poly I:C (TLR3 ligand) without affecting other pathways, suggesting it could help manage excessive macrophage activation during viral infections.
Background: Sustained type I interferon (IFN) activation Toll-like receptor (TLR) 3, 7 and 9 signaling has been reported to play a pivotal role in the development of lupus nephritis (LN). Although type I IFN activation has been shown to induce interferon-stimulated genes (ISGs) expression in systemic lupus erythematosus, the implication of ISGs expression in intrinsic glomerular cells remains largely unknown.
Methods: We treated cultured human glomerular endothelial cells (GECs) with polyinosinic-polycytidylic acid (poly IC), R848, and CpG (TLR3, TLR7, and TLR9 agonists, respectively) and analyzed the expression of DExD/H-Box Helicase 60 (DDX60), a representative ISG, using quantitative reverse transcription-polymerase chain reaction and western blotting.