synthesis of phospholipids with yeast phospholipase B, a phospholipid deacylating enzyme.

The gene encoding the phospholipid deacylation enzyme, phospholipase B (Sc), was successfully expressed in . The enzyme (Scplb1p) was engineered to have a histidine-tag at the C-terminal end and was purified by metal (Ni) affinity chromatography. Enzymatic properties, optimal pH, and substrate specificity were similar to those reported previously.