Publications by authors named "Takamichi Ohsaki"
Biol Pharm Bull
December 2006
Article Synopsis
- A recombinant rat aminopeptidase-B (Ap-B) was produced in E. coli as a GST fusion protein and purified through specific chromatography methods.
- Metal-substituted variants of Ap-B (Co(II)- and Cu(II)-Ap-B) were created, with Co(II)-Ap-B showing activity similar to the original enzyme, while Cu(II)-Ap-B demonstrated significantly lower activity.
- The study measured dissociation constants for zinc and cobalt in the enzymes and reported EPR parameters for Cu(II)-Ap-B, highlighting its distorted tetrahedral coordination geometry similar to other copper-enzyme complexes.
