GlcNAc6ST-1 regulates sulfation of N-glycans and myelination in the peripheral nervous system.

Highly specialized glial cells wrap axons with a multilayered myelin membrane in vertebrates. Myelin serves essential roles in the functioning of the nervous system. Axonal degeneration is the major cause of permanent neurological disability in primary myelin diseases.

Detection of N-glycans on small amounts of glycoproteins in tissue samples and sodium dodecyl sulfate-polyacrylamide gels.

N-linked glycans harbored on glycoproteins profoundly affect the character of proteins by altering their structure or capacity to bind to other molecules. Specific knowledge of the role of N-glycans in these changes is limited due to difficulties in identifying precise carbohydrate structures on a given glycoprotein, which arises from the large amounts of glycoprotein required for N-glycan structural determination. Here, we refined a simple method to purify and detect trace amounts of N-glycans.

Stroop performance and difficulties of older adults using automatic teller machines.

This study examined the relationship between inhibition of older dults and difficulties using automatic teller machines. 22 older adults performed three transactions on an ATM simulator, and errors were recorded. Participants also performed the Stroop interference task to assess inhibition.

Evaluation of a medetomidine-midazolam combination for immobilizing and sedating Japanese monkeys (Macaca fuscata).

We clinically and clinicopathologically investigated the immobilizing and sedative effects of a medetomidine-midazolam (MM) combination in Japanese monkeys (Macaca fuscata) and its antagonism with atipamezole. MM (medetomidine, 60 microg/kg; midazolam, 0.3 mg/kg) was administered intramuscularly to each monkey (n = 11).