HexR Transcription Factor Contributes to pv. Virulence by Coordinating Type Three Secretion System Genes.

pv. () causes bacterial blight on cabbage. We previously conducted a screening for reduced virulence using Tn transposon mutants and identified one of the transcriptional factors, HexR, as a potential virulence factor.

Large-Scale Transposon Mutagenesis Reveals Type III Secretion Effector HopR1 Is a Major Virulence Factor in pv. .

Bacterial canker of kiwifruit caused by pv. (Psa) is a serious threat to kiwifruit production worldwide. Four biovars (Psa biovar 1; Psa1, Psa biovar 3; Psa3, Psa biovar 5; Psa5, and Psa biovar 6; Psa6) were reported in Japan, and virulent Psa3 strains spread rapidly to kiwifruit production areas worldwide.

Controlling stomatal aperture, a potential strategy for managing plant bacterial disease.

Bacterial blight of crucifers caused by Pseudomonas cannabina pv. alisalensis (Pcal) inflicts great damage on crucifer production. To explore efficient and sustainable strategies for Pcal disease control, we here investigated and screened for amino acids with reduced disease development.

pv. Virulence Factors Are Involved in Resistance to Plant-Derived Antimicrobials during Infection.

Bacteria are exposed to and tolerate diverse and potentially toxic compounds in the natural environment. While efflux transporters are generally thought to involve bacterial antibiotic resistance in vitro, their contributions to plant bacterial virulence have so far been poorly understood. pv.

Multiple virulence factors regulated by AlgU contribute to the pathogenicity of pv. in soybean.

pv. () causes bacterial blight of soybean. To identify candidate virulence factors, transposon-mediated mutational analysis of was carried out.

Covering Soybean Leaves With Cellulose Nanofiber Changes Leaf Surface Hydrophobicity and Confers Resistance Against .

Asian soybean rust (ASR) caused by , an obligate biotrophic fungal pathogen, is the most devastating soybean production disease worldwide. Currently, timely fungicide application is the only means to control ASR in the field. We investigated cellulose nanofiber (CNF) application on ASR disease management.

pv. TrpA Is Required for Virulence in Multiple Host Plants.

pv. () causes bacterial leaf spot and blight of Brassicaceae and Poaceae. We previously identified several potential virulence factors with transposon mutagenesis.

Coronatine Contributes to pv. Virulence by Overcoming Both Stomatal and Apoplastic Defenses in Dicot and Monocot Plants.

pv. is a causative agent of bacterial blight of crucifers including cabbage, radish, and broccoli. Importantly, pv.

Acibenzolar-S-Methyl Activates Stomatal-Based Defense Systemically in Japanese Radish.

Acibenzolar-S-methyl (ASM) is a well-known plant activator, which is a synthetic analog of salicylic acid (SA). Recently, copper fungicides and antibiotics are major strategies for controlling bacterial diseases. However, resistant strains have already been found.

Flood inoculation of seedlings on culture medium to study interactions between pv. and kiwifruit.

Bacterial canker of kiwifruit caused by pv. (Psa) is a serious threat to kiwifruit production. Highly virulent strains of Psa biovar3 (Psa3) have spread rapidly to kiwifruit production areas worldwide.

Transposon mutagenesis reveals pv. optimizes its virulence factors for pathogenicity on different hosts.

pv. (), which causes bacterial blight disease of Brassicaceae, is an economically important pathogen worldwide. To identify genes involved in pathogenesis, we conducted a screen for 1,040 individual KB211 Tn mutants with reduced virulence on cabbage plants using a dip-inoculation method.

The SAL-PAP Chloroplast Retrograde Pathway Contributes to Plant Immunity by Regulating Glucosinolate Pathway and Phytohormone Signaling.

Chloroplasts have a crucial role in plant immunity against pathogens. Increasing evidence suggests that phytopathogens target chloroplast homeostasis as a pathogenicity mechanism. In order to regulate the performance of chloroplasts under stress conditions, chloroplasts produce retrograde signals to alter nuclear gene expression.

Flood-inoculation Method in .

pv. strain DC3000 ( DC3000), which causes bacterial speck disease of tomato, has been used as a model pathogen because of its pathogenicity on . Here, we demonstrate a rapid and reliable flood-inoculation method based on young seedlings grown on one-half strength MS medium.

NADPH-dependent thioredoxin reductase C plays a role in nonhost disease resistance against Pseudomonas syringae pathogens by regulating chloroplast-generated reactive oxygen species.

Chloroplasts are cytoplasmic organelles for photosynthesis in eukaryotic cells. In addition, recent studies have shown that chloroplasts have a critical role in plant innate immunity against invading pathogens. Hydrogen peroxide is a toxic by-product from photosynthesis, which also functions as a signaling compound in plant innate immunity.

Jasmonate ZIM-domain (JAZ) protein regulates host and nonhost pathogen-induced cell death in tomato and Nicotiana benthamiana.

The nonhost-specific phytotoxin coronatine (COR) produced by several pathovars of Pseudomonas syringae functions as a jasmonic acid-isoleucine (JA-Ile) mimic and contributes to disease development by suppressing plant defense responses and inducing reactive oxygen species in chloroplast. It has been shown that the F-box protein CORONATINE INSENSITIVE 1 (COI1) is the receptor for COR and JA-Ile. JASMONATE ZIM DOMAIN (JAZ) proteins act as negative regulators for JA signaling in Arabidopsis.

NTRC and chloroplast-generated reactive oxygen species regulate Pseudomonas syringae pv. tomato disease development in tomato and Arabidopsis.

Coronatine (COR)-producing pathovars of Pseudomonas syringae, including pvs. tomato, maculicola, and glycinea, cause important diseases on tomato, crucifers, and soybean, respectively, and produce symptoms with necrotic lesions surrounded by chlorosis. The chlorosis is mainly attributed to COR.

Arabidopsis seedling flood-inoculation technique: a rapid and reliable assay for studying plant-bacterial interactions.

Background: The Arabidopsis thaliana-Pseudomonas syringae model pathosystem is one of the most widely used systems to understand the mechanisms of microbial pathogenesis and plant innate immunity. Several inoculation methods have been used to study plant-pathogen interactions in this model system. However, none of the methods reported to date are similar to those occurring in nature and amicable to large-scale mutant screens.

Involvement of SGT1 in COR-mediated signal transduction pathway leading to disease symptom development.

Pseudomonas syringae pv. tomato DC3000 (Pst DC3000), that causes bacterial speck disease on tomato, produces a non-host-specific virulence effector, coronatine (COR). COR functions as a jasmonic acid (JA)-isoleucine mimic in planta and has multiple roles in the pathogenicity of Pst DC3000.

SGT1 contributes to coronatine signaling and Pseudomonas syringae pv. tomato disease symptom development in tomato and Arabidopsis.

• Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) causes an economically important bacterial speck disease on tomato and produces symptoms with necrotic lesions surrounded by chlorosis. The chlorosis is mainly attributed to a jasmonic acid (JA)-isoleucine analogue, coronatine (COR), produced by Pst DC3000.

Involvement of coronatine-inducible reactive oxygen species in bacterial speck disease of tomato.

pv. DC3000 ( DC3000) produces a chlorosis-inducing phytotoxin coronatine (COR), which has multiple virulence functions in planta. One of the hallmarks of bacterial speck disease on tomato leaves is the formation of necrotic lesions surrounded by chlorosis.

The phytotoxin coronatine induces light-dependent reactive oxygen species in tomato seedlings.

The phytotoxin coronatine (COR), which is produced by Pseudomonas syringae pv. tomato DC3000 (DC3000), has multiple roles in virulence that lead to chlorosis and a reduction in chlorophyll content. However, the physiological significance of COR-induced chlorosis in disease development is still largely unknown.

Pathogenicity of Pseudomonas syringae pv. tomato on tomato seedlings: phenotypic and gene expression analyses of the virulence function of coronatine.

Bacterial speck disease, which is caused by Pseudomonas syringae pv. tomato, is an economically important disease on tomato. In the present study, we show that P.