Regeneration of gingival tissue using in situ tissue engineering with collagen scaffold.

Objective: The aim of the study was to evaluate 2 types of collagen scaffold for gingival regeneration.

Study Design: Two types of collagen scaffolds, CS-pH7.4 and CS-pH3.

Optimal dehydrothermal processing conditions to improve biocompatibility and durability of a weakly denatured collagen scaffold.

Collagen scaffolds are essential for tissue regeneration; however, preprocessing of these scaffolds is necessary because of their poor mechanical properties. The aim of this study was to determine the optimal condition for preparing a collagen scaffold with biocompatibility and durability. An atelocollagen fiber suspension was made and stored at -10°C in a container that could be cooled from the bottom to provide an orientation perpendicular to the collagen fiber and facilitate cell infiltration into the scaffold.