Using a Relative Quantitative Proteomic Method to Identify Differentially Abundant Proteins in Biovar 3 and M5-90.

Brucellosis, caused by spp., is one of the most widespread bacterial zoonoses worldwide. Vaccination is still considered the best way to control brucellosis.

[Characterization of a recombinant goatpox virus expressing Orfv F1L gene].

Objective: In order to establish the vaccine against the contagious ecthyma, we constructed and characterized recombinant goatpox virus expressing F1L protein of Orf virus.

Methods: The F1L gene was amplified and cloned into the vector pUC-TK12 carrying the LacZ gene and a bidirectional promoter. With the help of lipidosome, the recombinant plasmid pTL-F1L was transfected into the BHK-21 cells, which had been infected by Gpv.