Dietary whey hydrolysate with exercise alters the plasma protein profile: a comprehensive protein analysis.

Objective: It has been shown that dietary whey protein accelerates glucose uptake by altering glycoregulatory enzyme activity in skeletal muscle. In the present study, we investigated the effect of dietary whey protein on endurance and glycogen resynthesis and attempted to identify plasma proteins that reflected the physical condition by a comprehensive proteomics approach.

Methods: Male c57BL/6 mice were divided into four groups: sedentary, sedentary with whey protein hydrolysate, exercise, and exercise with whey protein hydrolysate.

Preparation of proteins from different organs of Japanese morning glory by an in vivo electro-elution procedure.

An electro-elution procedure has been employed efficiently to collect proteins from stem segments, young leaves and roots of the Japanese morning glory. Electrophoretic conditions were optimised, including the size of segments (10-30 mm), the strength of the current for electro-elution (2.5-10 mA), and the exposure time of electro-elution (2-12 h).