Emergence and Persistence of High-Risk Clones Among MDR and XDR at a Brazilian Teaching Hospital.

Dissemination of carbapenem-resistant is currently one of the priority themes discussed around the world, including in Brazil, where this pathogen is considered endemic. A total of 107 carbapenem-resistant (CRAB) isolates were collected from patients with bacteraemia attended at a teaching hospital in Brazil from 2008 to 2014. From these samples, 104 (97.

Evaluation of MALDI-TOF VITEKMS and VITEK 2 system for the identification of Staphylococcus saprophyticus.

Aim: To compare two identification methods for coagulase-negative staphylococci (CoNS) isolated from patients with urinary tract infections, VITEK 2 and MALDI-TOF VITEKMS, with genotypic identification by internal transcribed spacer PCR (ITS-PCR).

Results: A total of 217 CoNS isolates were studied. Agreement of the VITEK 2 system with ITS-PCR was 84.

Detection of the mecA gene and identification of Staphylococcus directly from blood culture bottles by multiplex polymerase chain reaction.

Introduction: Staphylococcus spp. - both S. aureus, including methicillin-resistant strains (MRSA) and coagulase negative staphylococci (CoNS) - are relevant agents of healthcare-associated infections.