Induction of Salivary Gland-Like Tissue by Induced Pluripotent Stem Cells In Vitro.

Background: To investigate the in vitro induction of salivary gland-like tissue by ips cells in an interferon regulatory factor 6 (IRF6) overexpression and parotid conditioned medium environment.

Methods: Urine-derived ips cells were isolated, identified, transfected with IRF6 and cultured in parotid conditioned medium to induce ips cells into salivary gland differentiation, morphological changes of ips cells were observed, CCK-8 was used to determine the cell proliferation efficiency and transcriptome sequencing was used to detect the expression of genes related to parotid gland formation.

Results: Immunofluorescence staining showed that the isolated ips cells were positive for NANOG, SSEA4 and OCT4 and had embryonic-like stem cell characteristics; CCK-8 showed that there was no statistical difference in the proliferation efficiency between the IRF6 induced group and the simple induced group after induction of ips cells into salivary glands.

Diagnostic significance of magnetic resonance imaging in distinguishing temporomandibular disorders: a retrospective chart review.

Background: This study was to evaluate the diagnostic significance of Magnetic Resonance Imaging (MRI) in distinguishing temporomandibular disorders (TMD).

Methods: A total of 684 patients with TMD were included in the study. Diagnosis for TMD was conducted according to the international criteria.