Inhibition of direct-electron-transfer-type bioelectrocatalysis of bilirubin oxidase by silver ions.

In enzyme-based biosensors, Ag eluted from the reference electrode inhibits the enzyme activity. Herein, to suppress the inhibition of bilirubin oxidase (BOD) by Ag, kinetic analysis was used to examine the effect of Ag on the activity of BOD. It was confirmed that the addition of Ag decreased the bioelectrocatalytic activity of BOD.