Publications by authors named "Taichi Shintani"
Purpose: Microglia, the brain's immune cells, play important roles in neuronal differentiation, survival, and death. The function of microglia is deeply related to the morphologies; however, it is too complex to observe conventionally and identify the condition of living microglia using optical microscopes. Herein, we proposed a new method to observe living cultured microglia and their reactivity to inflammation via the acoustic impedance mode of a scanning acoustic microscope.
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- The study presents a new method to estimate the reflection coefficient of ultrasonic waves from glial cells, which helps visualize acoustic impedance distribution.
- It addresses limitations of traditional deconvolution techniques by using both frequency and time-frequency domain approaches to avoid signal artifacts.
- The findings reveal that the nucleus is centrally located within the cell and has a lower acoustic impedance than the cytoskeleton, potentially aiding in cell monitoring for regenerative medicine and drug discovery.