Triterpene saponins from tea seed pomace ( Abel) and their cytotoxic activity on MCF-7 cells in vitro.

Triterpenoid saponins are the main active ingredients extracted from Abel. In this study, crude saponins (Tc) was extracted from tea seed pomace and purified to obtain total saponins (T0). We used a COSMOSIL C18-OPN to separate T0 into three fractions-highly polar saponins (T1), moderately polar saponins (T2), and weakly polar saponins (T3).

Oleiferasaponin A₂, a Novel Saponin from Abel. Seeds, Inhibits Lipid Accumulation of HepG2 Cells Through Regulating Fatty Acid Metabolism.

A new triterpenoid saponin, named oleiferasaponin A₂, was isolated and identified from defatted seeds. Oleiferasaponin A₂ exhibited anti-hyperlipidemic activity on HepG2 cell lines. Further study of the hypolipidemic mechanism showed that oleiferasaponin A₂ inhibited fatty acid synthesis by significantly down-regulating the expression of and FAS protein, while dramatically promoting fatty acid β-oxidation by up-regulating the expression of and ACOX-1 protein.

Cytotoxic and Hypoglycemic Activity of Triterpenoid Saponins from Camellia oleifera Abel. Seed Pomace.

One new and three known triterpenoid saponins were isolated and identified from seeds through IR, NMR, HR-ESI-MS and GC-MS spectroscopic methods, namely oleiferasaponin A₃, oleiferasaponin A₁, camelliasaponin B₁, and camelliasaponin B₂. The structure of oleiferasaponin A₃ was elucidated as 16α-hydroxy-21β--angeloyl-22α--cinnamoyl-23α-aldehyde-28-dihydroxymethylene-olean-12-ene-3β--[β-d-galactopyranosyl-(1→2)]-[β-d-xylopyranosyl-(1→2)-β-d-galactopyranosyl-(1→3)]-β-d-gluco-pyranosiduronic acid. Camelliasaponin B₁ and camelliasaponin B₂ exhibited potent cytotoxic activity on three human tumour cell lines (human lung tumour cells (A549), human liver tumour cells (HepG2), cervical tumour cells (Hela)).