Formalin and ferric chloride inactivated Pasteurella multocida type a adjuvanted with bacterial DNA and alum as a new vaccine candidate in sheep pasteurellosis.

The aim of the present study was to investigate humoral and cellular immune responses in sheep inoculated with inactivated P. multocida antigen with alum and bacterial DNA adjuvant by identifying IgG and cytokines from serum and cell culture. Sheep were immunized with iron and formalin-inactivated antigens at an interval of 2 weeks.

Isolation of the various serotypes of Mannheimia haemolytica and preparation of the first vaccine candidate in Iran.

Background: Mannheimia haemolytica is one of the main agents of domestic pneumonic mannheimiosis, but a proper vaccine has not been explored in IRAN.

Methods And Results: 362 lung and nasal samples from sick domestic animal were detected by culture and PCR methods. Totally, 71 M.

Toxin typing of Associated with Enterotoxaemia in Sheep in Fars Province.

is implicated in the etiology of some diseases including fatal enterotoxaemia. Determining dominant toxin types of this microorganism can be helpful in epidemiologic surveys and the formulation of more proper vaccines. To understand the pathogenicity of this bacterium, it seems necessary to describe the toxin and virulence genes content of strains involved in enterotoxaemia and other associated diseases.

Pasteurella multocida inactivated with ferric chloride and adjuvanted with bacterial DNA is a potent and efficacious vaccine in Balb/c mice.

Purpose: Pasteurella multocida (P. multocida) is a principal pathogen of domestic animals and an opportunistic pathogen of humans. It is the causative agent of pneumonia and haemorrhagic septicaemia in cattle, sheep and goats, fowl cholera in chickens and progressive atrophic rhinitis in swine.

Molecular Analysis of rs2070744 and rs1799983 Polymorphisms of NOS3 Gene in Iranian Patients With Multiple Sclerosis.

Introduction: Multiple Sclerosis (MS) is a disease of central nervous system that mainly causes lesions or plaques in the spinal cord and brain. The purpose of this study was to analyze the relation between c.-813C>T (rs2070744) and c.

Genotypic analysis of virulence genes and antimicrobial profile of diarrheagenic Escherichia coli isolated from diseased lambs in Iran.

The aim of the present study was to determine the analysis of virulence genes and antimicrobial profile of diarrheagenic Escherichia coli isolated from diseased lambs. Two hundred ninety E. coli isolates were recovered from 300 rectal swabs of diarrheic lambs and were confirmed by biochemical tests.

Classification, capsular PCR typing and genetic diversity of Pasteurella multocida isolated from sheep and goats in Iran using Bam HI, Hind III restriction endonuclease enzymes by PCR-RFLP.

The aim of this study was to differentiatePasteurella multocidafrom ovine and caprine isolates in Iran by PCR-RFLP generated by restriction endonuclease enzymes Bam HI and Hind III. Among the isolates, 92.3% was identified as capsular type A and 7.

Prevalence of O157:H7 and non-O157 E. coli in Iranian domestic sheep.

The aim of the present study was the isolation of both E. coli O157 and non-O157 in sheep. Verotoxins (VT) 1, 2 and eae genes were tested for this propose.

First molecular detection of group A rotavirus in urban and hospital sewage systems by nested-RT PCR in Shiraz, Iran.

Background: Group A rotaviruses are the most significant cause of acute gastroenteritis in children worldwide. Rotaviruses are shed in high numbers and dispersed widely throughout bodies of water in the environment. This represents a significant health hazard for humans, mainly due to the stability of the viruses during wastewater treatment processes.

Evaluation of Cross Immunity and Histopathological Findings in Experimentally Infected BALB/c Mice with Neospora caninum and Besnoitia caprae.

Background: Caprine besnoitiosis is an economically important disease of goats. Neospora caninum, another coccidian parasite of worldwide distribution, infects several animal species and is a major cause of abortion in cattle. Combined infections of N.

Development of a disperse dye immunoassay technique for detection of antibodies against Neospora caninum in cattle.

In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of abortion were collected and tested by commercial ELISA kit and a standardized in-house dye immunoassay system. The positivity rate for the sera used in this study was 34.

Seroprevalence of Neospora spp. in Horses in North East of Iran.

Background: Neospora caninum, an obligate intracellular protozoan parasite, is recognized as a major cause of abortion in cattle, while limited information is presently available on the seroprevalence of Neospora antibodies in horses' worldwide. The aim of the present study was to determine serologic prevalence of Neospora infection in horses in Iran.

Methods: Sera from 150 horses from Mashhad suburb in Razavi Khorasan Province, northeast Iran were examined for antibodies to Neospora spp.

Detection of enterotoxigenic K99 (F5) and F41 from fecal sample of calves by molecular and serological methods.

Enterotoxigenic (ETEC) is one of the major causes of neonatal calf diarrhea. Almost all ETEC bacteria are known to adhere to receptors on the small intestinal epithelium via their fimbriae, (F5 (K99) and F41).This study was undertaken to investigate the phenotypic and genotypic screening of virulence genes in K99 and F41.

Prevention by rat amniotic fluid of adhesions after laparatomy in a rat model.

Background: Adhesion formation after abdominal surgery (especially multiple operations) is still a major cause of morbidities such as infertility, pain, bowel obstruction, difficult reoperation and other complications. Our aim was to investigate the ability of rat amniotic fluid to prevent adhesion formation after laparatomy in rats.

Methods: This experimental trial was conducted in 20 pregnant rats randomly assigned to two groups of 10 animals each.

Standardization of the outbred BALB/c mice as a suitable animal model for Besnoitia caprae studies.

The Apicomplexan parasite Besnoitia caprae infects wild and domestic goats. Knowledge on Besnoitia caprae specially an optimized animal model is sparse. Experimental infections with tachyzoites of BC-Pars obtained from BALB/c mice were conducted in outbred mice to determine the infectivity and LD50 of Besnoitia caprae.

Probiotic inhibits the cytopathic effect induced by Escherichia coli O157:H7 in Vero cell line model.

Aims: The effectiveness of four strains of Bifidobacteria against enterohemorrhagic Escherichia coli O157:H7 infection was studied using a Vero cell model.

Methods And Results: E. coli O157 was inoculated on the Vero cell line before and after treatment with probiotic.

Contamination of sheep carcasses with verocytotoxin producing Escherchia coli during slaughtering.

Verocytotoxin producing Escherchia coli (VTEC) infection has been associated with diarrhoea. Sheep, like other ruminants, appear to have VTEC in their feces and are regarded as natural reservoirs of these pathogens. As contaminated sheep products can serve as a risk factor, their role as a food safety threat should be considered.

Prevalence and distribution of the stx, stx genes in Shiga toxin producing E. coli (STEC) isolates from cattle.

Background And Objectives: Shiga toxin-producing Escherichia coli (STEC) strains are human pathogens linked to hemorrhagic colitis and hemolytic uremic syndrome. Shiga toxins (Stx1 and Stx2) are the major virulence factors of these strains. The aim of this work was to study the prevalence and distribution of stx(1) and stx(2) gene in E.

Detection and frequency of Stx2 gene in Escherichia coli O157 and O157:H7 strains isolated from sheep carcasses in Shiraz-Iran.

Enterohaemorrhagic Escherichia coli constitute a subset of serotypes (E. coli O157 and some other serogroups) of Shiga toxin-producing E. coli firmly associated with severe human illnesses like bloody diarrhoea and haemolytic uraemic syndrome.