Cucumis sativus (cucumber) was tested to assess an ecotoxicity in soils contaminated by the heavy metals copper (Cu), cadmium (Cd), and lead (Pb) separately and in combinations. The toxicity endpoint was plant growth, which was measured as shoot and root lengths after 5 day exposure. Sum of toxic unit (TU) at 50% inhibition for the mixture (EC50mix) was calculated from the dose (TU-based)-response relationships by the Trimmed Spearman-Karber method.
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