Genome-Wide In Silico Analysis of Leucine-Rich Repeat -Genes in : Classification and Expression Insights.

Background: Resistance () genes are crucial for defending Perilla against pathogens like anthracnose, downy mildew, and phytophthora blight. Nucleotide-binding site leucine-rich repeat (NBS-LRR) genes, the largest -gene family, play a central role in immunity. This study aimed to identify and characterize NBS-LRR genes in 'Jeju17'.

Complete genome sequence of type A strain (X58540) isolated from cattle feces.

The full genome sequence of type A strain X58540 found in fecal samples from cattle exhibiting clinical symptoms of chronic botulism is presented here. The genome of X58540 consists of 2,371,631 bp with a GC content of 28.17% and is predicted to include 2,157 coding sequences.

Comparative analysis of immunogenicity for viral hemorrhagic septicemia virus (VHSV) vaccines inactivated by different methods.

Viral hemorrhagic septicemia virus (VHSV) belonging to the genus Novirhabdovirus and family Rhabdoviridae is a major viral disease that causes economic loss in the olive flounder (Paralichthys olivaceus). In this study, we produced formalin-, binary ethylenimine (BEI)-, β-propiolactone (BPL)-, and heat-treated, inactivated VHSV vaccines; and observed the glycoprotein changes in the inactivated VHSV vaccines and confirmed their correlation with vaccine efficacy. The control group showed a 100 % cumulative mortality rate, but the groups immunized with formalin- and BPL-inactivated VHSV vaccines had high survival rates of 80 % and 90 %, and the virus copy numbers was significantly low.

Effect of diet changes in benthic ecosystems owing to climate change on the physiological responses of Turbo sazae in waters around Jeju Island, Korea.

The benthic ecosystem in temperate regions is becoming barren owing to the replacement of macroalgae by calcareous algae as a result of climate change-induced increases in water temperature. The aim of this study was to observe how the top shell, Turbo sazae, an invertebrate that feeds on macroalgae, adapts to a benthic environment with a reduced macroalgae population owing to replacement by calcareous algae. Using tank experiments, the top shells were cultured for 18 weeks.

A Study on the Battery Recycling Process and Risk Estimation.

The demand for the use of secondary batteries is increasing rapidly worldwide in order to solve global warming and achieve carbon neutrality. Major minerals used to produce cathode materials, which are key raw materials for secondary batteries, are treated as conflict minerals due to their limited reserves, and accordingly, research on the battery recycling industry is urgent for the sustainable secondary battery industry. There is a significant risk of accidents because there is a lack of prior research data on the battery recycling process and various chemicals are used in the entire recycling process.

Nanoceria as a non-steroidal anti-inflammatory drug for endometriosis theranostics.

Endometriosis, the growth of endometrial-like tissue outside the uterus, causes chronic pain and infertility in 10 % of reproductive-aged women worldwide. Unfortunately, no permanent cure exists, and current medical and surgical treatments offer only temporary relief. Endometriosis is a chronic inflammatory disease characterized by immune system dysfunction.

Discovery of Spirosnuolides A-D, Type I/III Hybrid Polyketide Spiro-Macrolides for a Chemotherapeutic Lead against Lung Cancer.

Four new macrolides, spirosnuolides A-D (-, respectively), were discovered from the termite nest-derived sp. INHA29. Spirosnuolides A-D are 18-membered macrolides sharing an embedded [6,6]-spiroketal functionality inside the macrocycle and are conjugated with structurally uncommon side chains featuring cyclopentenone, 1,4-benzoquinone, hydroxyfuroic acid, or butenolide moieties.

Application of Image-Based Phenotyping for QTL Identification of Tiller Angle in Rice ( L.).

Rice tiller angle is a key agronomic trait that regulates plant architecture and plays a critical role in determining rice yield. Given that tiller angle is regulated by multiple genes, it is important to identify quantitative trait loci (QTL) associated with tiller angle. Recently, with the advancement of imaging technology for plant phenotyping, it has become possible to quickly and accurately measure agronomic traits of breeding populations.

High-Performance Pure Water-Fed Anion Exchange Membrane Water Electrolysis with Patterned Membrane via Mechanical Stress and Hydration-Mediated Patterning Technique.

Despite rapid advancements in anion exchange membrane water electrolysis (AEMWE) technology, achieving pure water-fed AEMWE remains critical for system simplification and cost reduction. Under pure water-fed conditions, electrochemical reactions occur solely at active sites connected to ionic networks. This study introduces an eco-friendly patterning technique leveraging membrane swelling properties by applying mechanical stress during dehydration under fixed constraints.

Peptide-Based Prodrug Approaches for Cancer Nanomedicine.

Peptide-based prodrugs, such as peptide-drug conjugates (PDCs), are currently being developed for cancer therapy. PDCs are considered single-component nanomedicines with various functionalities. The peptide moieties provide stability to the PDCs, which self-assemble into nanostructures in an aqueous medium.

MnO Nanowires with Sub-10 nm Thick Conjugated Microporous Polymers as Synergistic Triboelectric Materials.

MnO nanowires coated with conjugated microporous polymers (CMP) are applied as triboelectric energy harvesting materials. The tribopositive performance of the CMP shells is enhanced with the assistance of MnO nanowires (MnO NW), likely due to cationic charge transfer from the tribopositive CMP layers to the surface Mn and Mn species of MnO NW. This is supported by model studies.

Comparative genomic analysis of copepod humoral immunity genes with sex-biased expression in Labidocera rotunda.

Studies of innate immune system function in invertebrates have contributed significantly to our understanding of the mammalian innate immune system. However, in-depth research on innate immunity in marine invertebrates remains sparse. We generated the first de novo genome and transcriptome sequences of copepod Labidocera rotunda using Illumina paired-end data and conducted a comparative genome analysis including five crustaceans (four copepods and one branchiopod species).

Multilayered alginate-hydrogel-functionalized zeolite based on ionic cross-linking composed with a certain Ca and Mg ratio for ammonium adsorption.

To enhance ammonium removal in wastewater treatment, a novel adsorbent, multilayered ionic cross-linked alginate-hydrogel-metal-coated zeolite (Al-H-M-Z), was synthesized. Essential divalent cations, 2.60 mmol of Ca and Mg, were incorporated into Al-H-M-Z (10 g) in a Ca:Mg ratio of 0.

Exogenous Lactate Treatment Immediately after Exercise Promotes Glycogen Recovery in Type-II Muscle in Mice.

Recent studies suggest that lactate intake has a positive effect on glycogen recovery after exercise. However, it is important to verify the effect of lactate supplementation alone and the timing of glycogen recovery. Therefore, in this study, we aimed to examine the effect of lactate supplementation immediately after exercise on glycogen recovery in mice liver and skeletal muscle at 1, 3, and 5 h after exercise.

Machine learning model to predict rate constants for sonochemical degradation of organic pollutants.

In this study, machine learning (ML) algorithms were employed to predict the pseudo-1st-order reaction rate constants for the sonochemical degradation of aqueous organic pollutants under various conditions. A total of 618 sets of data, including ultrasonic, solution, and pollutant characteristics, were collected from 89 previous studies. Considering the difference between the electrical power (P) and calorimetric power (P), the collected data were divided into two groups: data with P and data with P.

Tumor cell loaded thermosensitive hydrogel for photodynamic therapy associated tumor antigens release.

Background: Immunotherapy is a powerful strategy for treating cancer and can be used to inhibit the post-surgical relapse of tumors.

Methods: To achieve this, a Cell@hydrogel was developed as a template using a mixture of CT26 tumor cells and Pluronic® F-127/gelatin.

Results: The proposed mixture has a solution-to-gelation functionality and vice versa.

Inclusion Effect of Various Levels of Jack Mackerel Meal in Olive Flounder () Diets Substituting 50% Fish Meal with Duck By-Product Meal on Growth and Feed Utilization.

This experiment was performed to evaluate the inclusion impact of various levels of jack mackerel meal (JMM) in olive flounder () feeds substituting 50% FM by duck by-product meal (DBM) on growth, feed availability, and economic efficiency. Seven experimental diets were prepared. The control (Con) diet contained 60% FM.

Interplay of valley, layer and band topology towards interacting quantum phases in moiré bilayer graphene.

In Bernal-stacked bilayer graphene (BBG), the Landau levels give rise to an intimate connection between valley and layer degrees of freedom. Adding a moiré superlattice potential enriches the BBG physics with the formation of topological minibands - potentially leading to tunable exotic quantum transport. Here, we present magnetotransport measurements of a high-quality bilayer graphene-hexagonal boron nitride (hBN) heterostructure.

Sialyllactose supplementation enhances sialylation of Fc-fusion glycoprotein in recombinant Chinese hamster ovary cell culture.

Sialylation during N-glycosylation plays an important role in the half-life of therapeutic glycoproteins in vivo and has sparked interest in the production of therapeutic proteins using recombinant Chinese hamster ovary (rCHO) cells. To improve the sialylation of therapeutic proteins, we examined the effect of sialyllactose supplementation on sialylation of Fc-fusion glycoproteins produced in rCHO cells. Two enzymatically-synthesized sialyllactoses, 3'-sialyllactose (3'-SL) and 6'-sialyllactose (6'-SL), were administered separately to two rCHO cell lines producing the same Fc-fusion glycoprotein derived from DUKX-B11 and DG44, respectively.

Comparative analysis of obstetric, perinatal, and neurodevelopmental outcomes following chorionic villus sampling and amniocentesis.

Background: The risks of invasive prenatal tests are reported in previous studies such as miscarriage, fetal anomalies, and bleeding. However, few compare short-term and long-term outcomes between invasive tests. This study aims to investigate obstetric, perinatal, and children's neurodevelopmental outcomes following chorionic villus sampling (CVS) or amniocentesis in singleton pregnancy.

Daily station-level records of air temperature, snow depth, and ground temperature in the Northern Hemisphere.

Air temperature (Ta), snow depth (Sd), and soil temperature (Tg) are crucial variables for studying the above- and below-ground thermal conditions, especially in high latitudes. However, in-situ observations are frequently sparse and inconsistent across various datasets, with a significant amount of missing data. This study has assembled a comprehensive dataset of in-situ observations of Ta, Sd, and Tg for the Northern Hemisphere (higher than 30°N latitude), spanning 1960-2021.

Decoding Upconversion-Emitting Phase in Complex Composites Through Single-Particle-Level Upconversion Imaging and Density Functional Theory Calculations.

The crystal structure and phase stability of a host lattice plays an important role in efficient upconversion phenomena. In stable hosts, lanthanides doping should not generally change the crystal structure of the host itself. But when phase of a system drastically changes after lanthanide doping resulting in multiple phases, accurate identification of upconverting phase remains a challenge.