Publications by authors named "Tae-Young Kil"
Article Synopsis
- Amniotic fluid mesenchymal stem cells (AF-MSCs) are derived from fetal tissue and are recognized for their ability to self-renew and differentiate into various cell types.
- The study focused on analyzing their biological characteristics, including their morphology, growth potential, genetic makeup, and ability to develop into different cell types like fat, cartilage, and bone cells.
- Findings indicate that AF-MSCs maintain their stability and safety over long-term culture, making them a promising source for equine cell therapy due to their high reliability and low endotoxin levels.
Spinal Cord Injury (SCI) refers to complete or incomplete damage to the spinal cord, which comprises the central nervous system. SCI in dogs, like humans, is mostly caused by external trauma, and the degree of impact is dependent on the location of the injury in the spine. Stem cell therapy is a promising avenue for SCI research.
View Article and Find Full Text PDFBackground: Canine cloning technology based on somatic cell nuclear transfer (SCNT) combined with genome-editing tools such as CRISPR-Cas9 can be used to correct pathogenic mutations in purebred dogs or to generate animal models of disease.
Results: We constructed a CRISPR-Cas9 vector targeting canine DJ-1. Genome-edited canine fibroblasts were established using vector transfection and antibiotic selection.
Article Synopsis
- Somatic cell nuclear transfer (SCNT) is a common but costly and inefficient method used for cloning animals, leading researchers to explore the more straightforward handmade cloning (HMC) technique.
- The modified handmade cloning (mHMC) technique aims to reduce costs and improve efficiency by using basic lab equipment for porcine embryo cloning, while addressing issues like oocyte wastage and mitochondrial heteroplasmy.
- Results indicate that while mHMC shows comparable overall developmental competence to SCNT, it offers significant advantages in terms of simplicity and cost-effectiveness, suggesting it could be a viable alternative for porcine cloning.
Cumulus-oocyte complexes (COCs), which contain immature oocytes, are matured for in vitro embryo production. Oocyte and cumulus cells are then separated using hyaluronidase. To date, there have only been a few reported cases of the toxic effects of hyaluronidase on porcine oocytes.
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