Promotion of bone regeneration on titanium implants through a chemical treatment process using calcium phosphate slurry: Microscopic analysis, cellular response, and animal experiment.

The present study provides scientific evidence that a new chemical treatment process using calcium phosphate slurry promotes bone regeneration on titanium (Ti) implants. The material's surface modified by the treatment was analyzed using microscopic observation and the bone regeneration efficacy was evaluated both in vitro and in vivo. Formation of a thin hydroxyapatite layer with a thickness of about 50 nm and an increase of surface roughness were confirmed by microscopic observations.

A case report of a patient in whom antibodies against the 2009 pandemic influenza A/H1N1 virus have been present since June 1999.

A 69-y-old man with a history of hepatitis C since May 1985 and his 6 healthy immediate relatives were examined for hemagglutination inhibition antibodies against 2009 pandemic influenza A/H1N1 virus. This patient had a hemagglutination inhibition antibody titer of 640 against 2009 pandemic influenza A/H1N1 virus in a serum sample collected on July 4, 1999, and the antibody titers fluctuated between 40 and 320 in serum samples collected after 1999. The fluctuations in hemagglutination inhibition antibody titers against pandemic 2009 influenza A/H1N1 virus were not consistent with his history of seasonal influenza, and our results suggest a relationship to his vaccination with seasonal trivalent inactivated influenza vaccine.

Carbon nanohorns accelerate bone regeneration in rat calvarial bone defect.

A recent study showed that carbon nanohorns (CNHs) have biocompatibility and possible medical uses such as in drug delivery systems. It was reported that some kinds of carbon nanomaterials such as carbon nanotubes were useful for bone formation. However, the effect of CNHs on bone tissue has not been clarified.

Vascular cell-like potential of undifferentiated ligament fibroblasts to construct vascular cell-specific marker-positive blood vessel structures in a PI3K activation-dependent manner.

Objective: To evaluate whether fibroblasts derived from periodontal ligament retain the ability to differentiate into putative vascular cells and construct vascular cell-specific marker-positive blood vessel structures. We also evaluated the morphological features of the structure and investigated the intracellular molecular mechanism underlying the angiogenic activity of these cells.

Methods: Single cell-derived cultures (SCDCs) were established from primary rat ligament fibroblast cultures, and their expression of ligament cell-, mesenchymal stem cell- and vascular cell-specific markers was evaluated by RT-PCR and immunocytochemistry.

Fabrication of porous chitosan/hydroxyapatite nanocomposites: their mechanical and biological properties.

We fabricated novel chitosan/hydroxyapatite (HAp) nanocomposites with porous structure by the co-precipitation and porogen leaching method. SEM observation confirmed that the porous chitosan/HAp nanocomposites with 60.6% and 87.

Clinical value of genetically diagnosed lymph node micrometastasis for patients with oral squamous cell carcinoma.

Background: The purpose of this study was to investigate the incidence and clinical significance of genetically diagnosed lymph node micrometastasis for patients with oral squamous cell carcinoma (SCC).

Methods: A total of 495 lymph nodes obtained from 21 patients with primary oral SCCs that had p53 mutations were examined for corresponding p53 mutations in lymph nodes using mutant allele-specific amplification (MASA).

Results: Among 476 histologically negative nodes, 44 were scored as positive for metastasis by MASA.

The effect of vitamin K2 on bone metabolism in aged female rats.

Reactive oxygen species (ROS) may contribute to aging and osteoporosis resulting from marked decreases in plasma antioxidants in aged osteoporotic women. On the other hand, high-dose vitamin K2 (menaquinone-4: menatrenone, MK-4) supplementation has been reported to reduce ovariectomy-induced bone loss in rats and to decrease osteoporotic fracture in postmenopausal women. However, the mechanism by which vitamin K2 prevents osteoporosis is unclear.

Five patients with localized facial eruptions associated with Gianotti-Crosti syndrome caused by primary Epstein-Barr virus infection.

Five infants exhibited an exclusively facial rash associated with Gianotti-Crosti syndrome(GCS). On all patient cheeks, multiple erythematous papules were seen symmetrically. All patients tested positive for the immunoglobulin (Ig)M antibody against the Epstein-Barr viral capsid antigen, suggesting that a primary Epstein-Barr virus (EBV) infection caused localized facial rash, an alternative clinical picture of GCS.

Marginal resection for carcinoma of the mandibular alveolus and gingiva where radiologically detected bone defects do not extend beyond the mandibular canal.

Purpose: The study goal was to investigate the adequacy of marginal resection of the mandibular bone in cases of squamous cell carcinomas of the lower alveolus and gingiva where the radiologically detected bone defect does not extend beyond the mandibular canal.

Patients And Methods: Sixty-two patients who have had a mandibulectomy performed with or without preoperative radiotherapy and chemotherapy were the study subjects. This retrospective study investigated the correlation between the preoperative radiographic appearance and histopathologic findings of the excised tissue.

Bone metabolism and oxidative stress in postmenopausal rats with iron overload.

Osteoporosis is associated with many etiological causes such as nutrition, cytokines, hormones, and aging. Recently, reactive oxygen species (ROS) are considered to be responsible for the aging process and osteoporosis. We investigated the relationship between ROS and bone metabolism in young female and postmenopausal rats, by using dietary iron overload and several indices including bone metabolic markers, oxidative stress and antioxidant markers, and cytokines.

Radioresistance in oral squamous cell carcinoma with p53 DNA contact mutation.

Reliable variables to predict the radiosensitivity of each tumor have not been identified. Recent studies have demonstrated that specific regions of mutations within the core domain of p53 protein correlate with responses to chemotherapy and radiotherapy in some tumor types. In this study, we evaluated the relationship between specific p53 mutations and radiosensitivity in 49 patients with oral squamous cell carcinomas (SCCs) who underwent preoperative radiotherapy.

Mucinous adenocarcinoma of probable minor salivary gland origin.

Mucinous adenocarcinoma is characterized by large pools of extracellular mucin. The tumor has been reported in the nasal cavity, paranasal sinus, breast, colon, stomach, prostate, skin, and lung. Mucinous adenocarcinoma also arises as a primary tumor of the major salivary glands.