Theory about the Embryo Cryo-Treatment.

Background: To create hypothesis, which can give a logical explanation related to the benefits of freezing/thawing embryos. Cryopreservation is not only a technology used for storing embryos, but also a method of embryo treatment that can potentially improve the success rate in infertile couples.

Methods: From the analysis of multiple results in assisted reproductive technology, which have no satisfactory explanation to date, we found evidence to support a 'therapeutic' effect of the freezing/thawing of embryos on the process of recovery of the embryo and its subsequent implantation.

Properties of the Michael's adducts of the substituted 2-amino-5-oxonitriles.

A series of five substituted 2-amino-5-oxonitriles, obtained by the Michael reaction have been designed, synthesised, isolated and spectroscopically and structurally elucidated by means of single crystal X-ray diffraction, linear-polarized infrared (IR-LD) and UV-spectroscopy, (1)H NMR, mass spectrometry and thermal methods. Theoretical quantum chemical calculations were carried out, thus supporting the experimental optical properties and electronic structure of compounds studied.

[Endocrinology of in vitro maturation of oocytes].

In-vitro maturation (IVM) of oocytes is an alternative to conventional in-vitro fertilization (IVF) treatment. Significant progress has been made to improve pregnancy and implantation rates from in-vitro matured oocytes. However oocyte maturation in vitro is profoundly affected by culture conditions.

[Measurement of basal anti-Mullerian hormone level of healthy fertile women in Bulgaria].

Objective: To establish mean and reference range of basal serum Anti-Mullerian hormona (AMH) levels in a group of healthy fertile Bulgarian women.

Methods: The present report covers the results of 9 month's period in which 72 healthy fertile Bulgarian women, aged 20-40 years were selected to determinate a reference group of healthy fertile women with normal values of AMH. Blood samples were collected on early follicular phase of menstrual cycle (2-4 days).

[Anti-Mullerian hormone: a gonadal hormone with multiple diagnostics resources].

Anti-Mullerian hormone (AMH) is a gonadal hormone synthesized by granulose cells of the ovary and Sertoli cells of the testis. Anti-Mullerian hormone is used to facilitate the evaluation of intersex disorders and as a marker in some ovarian tumors or ovarian reserve assessment in the infertility cases. Serum levels of AMH hold objective information, which is useful in the clinical practice.

Prognostic value of some ovarian reserve tests in poor responders.

Background: The objective of this study was to determine the prognostic value of the basal estradiol (E2) and inhibin-B levels, the antral follicle count (AFC), and the clomiphene citrate challenge test (CCCT) of ovarian response in controlled ovarian hyperstimulation (COH), in an outcome with normal follicle-stimulating hormone (FSH) concentration in the early follicular phase of the menstrual cycle.

Methods: Fifty-two patients undergoing IVF treatment were included in the study. Blood samples were collected for assessment of basal E2, FSH, and inhibin-B levels.

[Measurement of basal inhibin B level in Bulgarian women of the childbearing age].

Objective: To establish mean and reference range of basal serum inhibin B levels in a group of healthy fertile Bulgarian women.

Methods: The present report covers the results of 9 month's period in which sixty-five healthy fertile Bulgarian women, aged 20-40 years were selected to determinate a reference group of healthy fertile women with normal values of inhibin B. Blood samples were collected on early follicular phase of menstrual cycle (2-4 days).

Mean ovarian diameter (MOD) as a predictor of poor ovarian response.

Purpose: To determine the predictive value of mean ovarian diameter of ovarian response in controlled ovarian hyperstimulation (COH), in outcome with normal FSH level in early follicular phase of menstrual cycle.

Methods: A prospective study established the prognostic value of the mean ovarian diameter of total 57 cases, including 17 patients undergoing IVF treatment with poor ovarian response and 40 patients (control group) with adequate ovarian response.

Results: The mean ovarian diameter (MOD) of patients in the cancelled group was significantly lower (19.

[Prognostic value of some hormonal and ultrasound ovarian reserve tests].

Objective: To determine the predictive value of basal estradiol, FSH and inhibin B levels, antral follicle count and mean ovarian diameter of ovarian response in controlled ovarian hyperstimulation (COH), in outcome with normal FSH concentration in early follicular phase of menstrual cycle.

Materials And Methods: Twenty nine patients undergoing IVF treatment. Blood samples were collected and transvaginal ultrasound was performed on early follicular phase of unstimulated cycle.