The oleaginous yeast Rhodosporidium toruloides engineered for biomass hydrolysate-derived (E)-⍺-bisabolene production.

The oleaginous yeast R. toruloides has been exploited for many bioproducts, including several terpenes, owing to its oleaginous nature and biomass inhibitor tolerance. Here, we built upon previous (E)-⍺-bisabolene work by iteratively stacking the complete mevalonate pathway from Saccharomyces cerevisiae onto a multicopy bisabolene synthase parent strain.