Potential Effects of the Different Matrices to Enhance the Polyphenolic Content and Antioxidant Activity in Gluten-Free Bread.

Gluten-related disorders, including celiac disease, wheat allergy, and non-celiac gluten sensitivity, have emerged as a significant phenomenon affecting people worldwide, with an estimated prevalence of nearly 5% globally. The only currently available treatment for this disease involves the exclusion of gluten from the diet, which is particularly challenging in the case of bakery products. Gluten-free bread (GFB) presents certain disadvantages when compared to traditional wheat bread, including inferior sensory attributes, technological characteristics, and lower protein and fiber content.

Cassava starch and chickpea flour pre-treated by microwave as a substitute for gluten-free bread additives.

There is an increasing demand for gluten-free products, which are regularly made by a combination of ingredients and additives. Microwave pre-treatment of gluten-free ingredients is an alternative to food additives because it may induce changes in protein and starch functional properties. In this context, this study aimed to apply microwave treatment in cassava starch and chickpea flour, analyzing their functional and thermal properties and their ability to substitute additives in gluten-free breads, comparing them to an additive-containing bread.

Evolution of the spontaneous sourdoughs microbiota prepared with organic or conventional whole wheat flours from South Brazil.

The purpose of this study was to compare the composition and stability of bacteria and fungi communities during the propagation of sourdoughs prepared with organic or conventional whole wheat (Triticum aestivum) flours from South Brazil. Sourdoughs were prepared and samples were collected during different fermentation times (0 to 216 h). Total DNA of sourdough samples were extracted and the 16S rRNA gene and Internal Transcribed Spacer region were sequenced by MiSeq-Illumina.

Antifungal properties of phosphatidylcholine-oleic acid liposomes encapsulating garlic against environmental fungal in wheat bread.

Liposomes have gained great interest in the food and pharmaceutical industry as colloidal carriers of bioactive compounds. In this work, liposomes of phosphatidylcholine (PC) and oleic acid (OA) encapsulating garlic extract (GE) were developed to determine its aptitude as antifungal agent in wheat bread. The influence of GE on the properties of liposomes were followed by determination of size, Zeta potential, Fourier transform infrared patterns (FTIR), morphology, differential scanning calorimetry (DSC) and thermogravimetric (TGA) techniques.

Alternative DNA secondary structure formation affects RNA polymerase II promoter-proximal pausing in human.

Background: Alternative DNA secondary structures can arise from single-stranded DNA when duplex DNA is unwound during DNA processes such as transcription, resulting in the regulation or perturbation of these processes. We identify sites of high propensity to form stable DNA secondary structure across the human genome using Mfold and ViennaRNA programs with parameters for analyzing DNA.

Results: The promoter-proximal regions of genes with paused transcription are significantly and energetically more favorable to form DNA secondary structure than non-paused genes or genes without RNA polymerase II (Pol II) binding.

DNA Replication Dynamics of the GGGGCC Repeat of the C9orf72 Gene.

DNA has the ability to form a variety of secondary structures in addition to the normal B-form DNA, including hairpins and quadruplexes. These structures are implicated in a number of neurological diseases and cancer. Expansion of a GGGGCC repeat located at C9orf72 is associated with familial amyotrophic lateral sclerosis and frontotemporal dementia.

Environmental and chemotherapeutic agents induce breakage at genes involved in leukemia-causing gene rearrangements in human hematopoietic stem/progenitor cells.

Hematopoietic stem and progenitor cells (HSPCs) give rise to all of the cells that make up the hematopoietic system in the human body, making their stability and resilience especially important. Damage to these cells can severely impact cell development and has the potential to cause diseases, such as leukemia. Leukemia-causing chromosomal rearrangements have largely been studied in the context of radiation exposure and are formed by a multi-step process, including an initial DNA breakage and fusion of the free DNA ends.

DNA secondary structure at chromosomal fragile sites in human disease.

DNA has the ability to form a variety of secondary structures that can interfere with normal cellular processes, and many of these structures have been associated with neurological diseases and cancer. Secondary structure-forming sequences are often found at chromosomal fragile sites, which are hotspots for sister chromatid exchange, chromosomal translocations, and deletions. Structures formed at fragile sites can lead to instability by disrupting normal cellular processes such as DNA replication and transcription.

G4 resolvase 1 tightly binds and unwinds unimolecular G4-DNA.

It has been previously shown that the DHX36 gene product, G4R1/RHAU, tightly binds tetramolecular G4-DNA with high affinity and resolves these structures into single strands. Here, we test the ability of G4R1/RHAU to bind and unwind unimolecular G4-DNA. Gel mobility shift assays were used to measure the binding affinity of G4R1/RHAU for unimolecular G4-DNA-formed sequences from the Zic1 gene and the c-Myc promoter.

Effect of the alkaline treatment on the ultrastructure of C-type starch granules.

The effect of alkaline treatment on the ultrastructure of C-type starch granules was investigated during the alkaline extraction of Araucaria angustifolia (pinhao) starch. The efficiency in protein removal was evaluated using intrinsic fluorescence and Kjeldahl's method. In parallel, morphological changes of starch granules were observed using scanning electron microscopy and atomic force microscopy.

Purification and properties of a keratinolytic metalloprotease from Microbacterium sp.

Aims: This study was developed to purify and to characterize a keratinolytic protease from the bacterium Microbacterium sp. strain kr10.

Methods And Results: Enzyme purification was carried out by sequential liquid chromatography on Sephadex G-100 and Q-Sepharose columns.

Characterization of a protease of a feather-degrading Microbacterium species.

Aims: To characterize a new feather-degrading bacterium.

Methods And Results: The strain kr10 producing a high keratinolytic activity when cultured on native feather broth was identified as Microbacterium sp., based on phenotypical characteristics and 16S rDNA sequence.

[Tibiotarsal and subtalar instability of the ankle. Importance of an original dynamic test in the treatment choice. Experimental study and clinical application].

Various ligamentoplasties are available allowing formation of new proprioceptive chains in cases of failure of physiotherapy. Most of these plasties sacrifice half or all of the fibulolateral tendon, causing stiffness of the subtalar joint. This is a too serious complication to accept for simple tibiotarsal instability: hence the importance of preoperative assessment to determine the type of plasty most appropriate for each type of instability.

[Reflections on a small series of cementless total hip prostheses].

We report on our series of 48 patients with uncemented hip prostheses, with an average follow-up of 3 years. The clinical results, according to the Merle d'Aubigné's classification, are comparable to those obtained with cemented prostheses except for transitory pain, very often localized to the lateral aspect of the thigh, which seems to be correlated with the bone adaptation to the implant. Radiological findings are valuable in the assessment of long-term results with uncemented prostheses.

[Osteosynthesis of lesions of the proximal femur using dynamic screw plates. Multicenter study: 1871 cases].

A series of 1871 lesions of the upper end of the femur were treated in 32 hospitals in Belgium using a dynamic hip screw. Unstable pertrochanteric fractures were present in 34.4%.

Transfer of functional insulin receptors to receptor-deficient target cells.

Purified human insulin receptors incorporated into phospholipid vesicles have previously been shown to retain insulin binding as well as insulin-stimulated beta-subunit autophosphorylating activity. These vesicles were used as a vehicle to transfer receptors to the insulin receptor-deficient Madin-Darby canine kidney (MDCK) cell line. Fusion of control [14C] dioleoylphosphatidylcholine-labeled phospholipid vesicles with MDCK cells was found to be dependent on both the amount of time and the concentration of polyethylene glycol used for fusion.

High-level expression of human insulin receptor cDNA in mouse NIH 3T3 cells.

In order to develop a simple, efficient system for the high-level expression of human insulin receptors in eukaryotic cells, a full-length human kidney insulin receptor cDNA was inserted into a bovine papilloma virus vector under the control of the mouse metallothionein promoter. After transfection of mouse NIH 3T3 cells with this construct, seven cell lines expressing insulin receptors were isolated; two cell lines had more than 10(6) receptors per cell. The cell line with the highest insulin binding (NIH 3T3 HIR3.

[Trapeziectomy in rhizarthrosis].

The purpose of this study is to determine the place of trapeziectomy among the various surgical treatments of osteoarthritis of the trapezio-metacarpal joint. We have had 25 cases with seemingly satisfactory results. Loss of strength, which is given as a frequent objection to this method, has not generally been found in our series.