Super-photostable organic dye for long-term live-cell single-protein imaging.

Organic dyes play a crucial role in live-cell imaging because of their advantageous properties, such as photostability and high brightness. Here we introduce a super-photostable and bright organic dye, Phoenix Fluor 555 (PF555), which exhibits an order-of-magnitude longer photobleaching lifetime than conventional organic dyes without the requirement of any anti-photobleaching additives. PF555 is an asymmetric cyanine structure in which, on one side, the indole in the conventional Cyanine-3 is substituted with 3-oxo-quinoline.

Nanoscale Magnetic Ordering Dynamics in a High Curie Temperature Ferromagnet.

Thermally driven transitions between ferromagnetic and paramagnetic phases are characterized by critical behavior with divergent susceptibilities, long-range correlations, and spin dynamics that can span kHz to GHz scales as the material approaches the critical temperature , but it has proven technically challenging to probe the relevant length and time scales with most conventional measurement techniques. In this study, we employ scanning nitrogen-vacancy center based magnetometry and relaxometry to reveal the critical behavior of a high- ferromagnetic oxide near its Curie temperature. Cluster analysis of the measured temperature-dependent nanoscale magnetic textures points to a 3D universality class with a correlation length that diverges near .

Synthesis and Characterization of UV-Curable Resin with High Refractive Index for a Luminance-Enhancing Prism Film.

A novel monomer, 9-bis[4-(2-hydroxyethoxy)phenyl]fluorene di(mercaptopropionate), with a highly refractive index, purity, and excellent UV-curable properties, is synthesized through an optimized Fischer esterification process, reacting 9,9-bis[4-(2-hydroxyethoxy)phenyl]fluorene with 3-mercaptopropionic acid. The structural characterization of this monomer is performed using Fourier-transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, high-performance liquid chromatography, and liquid chromatography-mass spectrometry. The synthesis conditions are optimized using a design-of-experiments approach.

A Randomized, Blinded, Vehicle-Controlled Dose-Ranging Study to Evaluate and Characterize Remdesivir Efficacy Against Ebola Virus in Rhesus Macaques.

Ebola virus (EBOV) causes severe disease in humans, with mortality as high as 90%. The small-molecule antiviral drug remdesivir (RDV) has demonstrated a survival benefit in EBOV-exposed rhesus macaques. Here, we characterize the efficacy of multiple intravenous RDV dosing regimens on survival of rhesus macaques 42 days after intramuscular EBOV exposure.

The association between the polymorphism of RAR related orphan receptor beta and the clinical manifestations of oral squamous cell carcinoma.

Oral squamous cell carcinoma (OSCC) affects a substantial proportion of the Asian population and is influenced by various genetic risk factors. The (), a regulator of the circadian rhythm, has been implicated in certain neoplasms. Accordingly, this study investigated the association between single-nucleotide polymorphisms and clinical manifestations of OSCC.

Signatures of ambient pressure superconductivity in thin film LaNiO.

Recently, the bilayer nickelate LaNiO has been discovered as a new superconductor with transition temperature T near 80 K under high pressure. Despite extensive theoretical and experimental work to understand the nature of its superconductivity, the requirement of extreme pressure restricts the use of many experimental probes and limits its application potential. Here, we present signatures of superconductivity in LaNiO thin films at ambient pressure, facilitated by the application of epitaxial compressive strain.

The Relationship of Pork Meat Consumption with Nutrient Intakes, Diet Quality, and Biomarkers of Health Status in Korean Older Adults.

Background: Pork meat is a widely consumed protein food with the potential to differentially affect health and nutritional status across social and cultural contexts.

Objectives: We evaluated the association between pork meat consumption and nutrient intake, diet quality, and biomarkers of health among older adults (age ≥ 65 years) in Korea.

Methods: Our analyses utilized dietary and health examination data from the 2016-2020 Korean National Health and Nutrition Examination Survey ( = 2068).

Persistent ferromagnetic ground state in pristine and Ni-doped FeGaTe flakes.

Room-temperature magnetism and its stability upon miniaturization are essential characteristics required for materials for spintronic devices and information storage. Among various candidates, FeGaTe stands out due to its high Curie temperature and strong perpendicular magnetic anisotropy (PMA), recently gaining large attention as one of the promising candidate materials for spintronics applications. In this study, we measured the thickness-dependent ferromagnetic properties of FeGaTe and (FeNi)GaTe (with x = 0.

Autofluorescence Virtual Staining System for H&E Histology and Multiplex Immunofluorescence Applied to Immuno-Oncology Biomarkers in Lung Cancer.

We extend the capabilities of virtual staining from AF to a different disease and stain modality. Our work includes newly developed virtual stains for H&E and a multiplex immunofluorescence panel (DAPI, PanCK, PD-L1, CD3, and CD8) for non-small cell lung cancer, which reproduce the key features of real stains.

Universal Spreading of Conditional Mutual Information in Noisy Random Circuits.

We study the evolution of conditional mutual information (CMI) in generic open quantum systems, focusing on one-dimensional random circuits with interspersed local noise. Unlike in noiseless circuits, where CMI spreads linearly while being bounded by the light cone, we find that noisy random circuits with an error rate p exhibit superlinear propagation of CMI, which diverges far beyond the light cone at a critical circuit depth t_{c}∝p^{-1}. We demonstrate that the underlying mechanism for such rapid spreading is the combined effect of local noise and a scrambling unitary, which selectively removes short-range correlations while preserving long-range correlations.

Antibiotic Treatment for 7 versus 14 Days in Patients with Bloodstream Infections.

Background: Bloodstream infections are associated with substantial morbidity and mortality. Early, appropriate antibiotic therapy is important, but the duration of treatment is uncertain.

Methods: In a multicenter, noninferiority trial, we randomly assigned hospitalized patients (including patients in the intensive care unit [ICU]) who had bloodstream infection to receive antibiotic treatment for 7 days or 14 days.

Machine Learning Algorithms to Predict the Risk of Rupture of Intracranial Aneurysms: a Systematic Review.

Purpose: Subarachnoid haemorrhage is a potentially fatal consequence of intracranial aneurysm rupture, however, it is difficult to predict if aneurysms will rupture. Prophylactic treatment of an intracranial aneurysm also involves risk, hence identifying rupture-prone aneurysms is of substantial clinical importance. This systematic review aims to evaluate the performance of machine learning algorithms for predicting intracranial aneurysm rupture risk.

Correlations between the long noncoding RNA MEG3 and clinical characteristics for diabetic kidney disease in type 2 diabetes mellitus.

Background And Aims: Diabetic kidney disease (DKD) is a common complication of type 2 diabetes mellitus (T2DM) that leads to systemic inflammation. Maternally expressed gene 3 (MEG3) is a tumor suppressor that is involved in inflammation regulation. The current study investigated the association between DKD and the prevalence of the single-nucleotide polymorphisms (SNPs) of MEG3.

Giant Uniaxial Magnetocrystalline Anisotropy in SmCrGe.

Magnetic anisotropy is a crucial characteristic for enhancing the spintronic device performance. The synthesis of SmCrGe single crystals through a high-temperature solution method has led to the determination of uniaxial magnetocrystalline anisotropy. Phase verification was achieved by using scanning transmission electron microscopy (STEM), powder, and single-crystal X-ray diffraction techniques.

Development and Field Test of Integrated Electronics Piezoelectric Accelerometer Based on Lead-Free Piezoelectric Ceramic for Centrifugal Pump Monitoring.

In this study, an Integrated Electronics Piezoelectric (IEPE)-type accelerometer based on an environmentally friendly lead-free piezoceramic was fabricated, and its field applicability was verified using a cooling pump owned by the Korea Atomic Energy Research Institute (KAERI). As an environmentally friendly piezoelectric material, 0.96(K,Na)NbO-0.

Principles of paralog-specific targeted protein degradation engaging the C-degron E3 KLHDC2.

PROTAC® (proteolysis-targeting chimera) molecules induce proximity between an E3 ligase and protein-of-interest (POI) to target the POI for ubiquitin-mediated degradation. Cooperative E3-PROTAC-POI complexes have potential to achieve neo-substrate selectivity beyond that established by POI binding to the ligand alone. Here, we extend the collection of ubiquitin ligases employable for cooperative ternary complex formation to include the C-degron E3 KLHDC2.

Impact of Cystic Fibrosis Transmembrane Conductance Regulator Modulators on Maternal Outcomes During and After Pregnancy.

Background: Cystic fibrosis (CF) transmembrane conductance regulator (CFTR) modulators are available to the majority of people with CF in the United States; little is known about pregnancy outcomes with modulator use. The aim of this retrospective study was to determine the impact of CFTR modulators on maternal outcomes.

Research Question: Does pregnancy differentially affect outcomes in female individuals with CF with and without CFTR modulator exposure?

Study Design And Methods: Data on pregnancies from 2010 to 2021 were collected from 11 US adult CF centers.

Visible and near-infrared modulating tungsten suboxide nanorods electrochromic films in acidic aqueous electrolytes.

Proton-based aqueous electrolytes can be used to achieve high performance electrochromic nanocrystal thin films due to their small ion size. However, acidic aqueous electrolyte systems have not yet been explored in near-infrared (NIR) absorbing plasmonic tungsten oxide nanocrystal films. Here, we demonstrate tungsten suboxide nanorod films with excellent visible and NIR modulation performance in the H-based aqueous electrolytes, thanks to their mesoporous structure, nanosized domains, and open tunnel structure.

Idiopathic intracranial hypertension in Asians: a retrospective dual-center study.

Background: There have been limited data on idiopathic intracranial hypertension (IIH) in Asians and there remain uncertainties whether a cerebrospinal fluid (CSF) pressure of 250 mm CSF is an optimum diagnostic cutoff. The aims of the present study included (1) characterization of IIH patients in Taiwan, (2) comparisons among different diagnostic criteria for IIH, and (3) comparisons between patients with CSF pressures of > 250 and 200-250 mm CSF.

Methods: This retrospective study involved IIH patients based on the modified Dandy criteria from two tertiary medical centers in Taiwan.