Publications by authors named "T van den Bos"
Optimization algorithms play an important role in method development workflows for gradient elution liquid chromatography. Their effectiveness has not been evaluated for chromatographic method development using standardized comparisons across factors such as sample complexity, chromatographic response functions (CRFs), gradient complexity, and application type. This study compares six optimization algorithms - Bayesian optimization (BO), differential evolution (DE), a genetic algorithm (GA), covariance-matrix adaptation evolution strategy (CMA-ES), random search, and grid search - for the development of gradient elution LC methods.
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- Monoclonal antibodies undergo various post-translational modifications that can affect their charge and quality, making it essential to assess these changes to ensure safety in pharmaceuticals.
- A newly developed capillary zone electrophoresis-mass spectrometry (CZE-MS) method allows for effective separation and identification of charge variants in monoclonal antibodies, utilizing a neutral static capillary coating and a volatile background electrolyte.
- This optimized method has shown reliable results in correlating charge variant profiles, enabling better assessment of mAb quality and facilitating the identification of variants that impact their therapeutic effectiveness.
Fasting hypoglycemia is a severe and incompletely understood symptom of various inborn errors of metabolism (IEM). Precision-cut liver slices (PCLS) represent a promising model for studying glucose production ex vivo. This study quantified the net glucose production of human and murine PCLS in the presence of different gluconeogenic precursors.
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- Polyesters are important in high-end industrial applications, but their complex chemical properties make them challenging to analyze effectively.
- A new analytical method combining normal-phase liquid chromatography, size-exclusion chromatography, ultraviolet-light spectroscopy, and high-resolution mass spectrometry allows for detailed characterization of polyesters, focusing on their end-group functionality and molecular weight distributions.
- This method enables comprehensive analysis of polyesters, providing crucial information on their chemical composition and properties that cannot be attained through traditional techniques, making it highly valuable for industrial applications.
A workplace violence prevention program targeting high-risk patients in emergency departments.
J Am Coll Emerg Physicians Open
August 2024
Objective: Patient violence in emergency departments (EDs) may be prevented with proactive mitigation measures targeting potentially violent patients. We aimed to evaluate the effects of two interventions guided by a validated risk-assessment tool.
Methods: A prospective interventional study was conducted among patients ≥10 years who visited two EDs in Michigan, USA, from October 2022 to August 2023.