Publications by authors named "T van Gorkom"
Article Synopsis
- The study looked at different testing methods for diagnosing Lyme disease, focusing on two strategies: standard (STTT) and modified (MTTT).
- Both testing strategies were used on patients with Lyme neuroborreliosis (LNB) and Lyme arthritis (LA) to see how well they worked.
- The results showed that the modified strategy was slightly better in finding Lyme disease without losing accuracy, making it a good choice for doctors to use.
Laboratory diagnosis of Lyme neuroborreliosis (LNB) is challenging, and validated diagnostic algorithms are lacking. Therefore, this retrospective cross-sectional study aimed to compare the diagnostic performance of seven commercial antibody assays for LNB diagnosis. Random forest (RF) modeling was conducted to investigate whether the diagnostic performance using the antibody assays could be improved by including several routine cerebrospinal fluid (CSF) parameters (i.
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- A retrospective study assessed the effectiveness of nine serological assays for diagnosing Lyme borreliosis (LB) using sera from 74 LB cases and 122 controls.
- Sensitivity of these tests varied significantly, with higher detection rates in cases like Lyme neuroborreliosis compared to early Lyme cases, and overall performance was adequate across both early and late forms of LB.
- The study concluded that while serological assays are essential for diagnosing LB, IgM testing did not enhance diagnostic accuracy beyond IgG testing, and a two-tier testing approach is recommended to minimize false positives.
Recent studies have shown elevated levels of the B-cell chemokine (C-X-C motif) ligand 13 (CXCL13) in the cerebrospinal fluid (CSF) of patients with early Lyme neuroborreliosis (LNB). In this retrospective study, we evaluated the diagnostic performance of the Quantikine CXCL13 enzyme-linked immunosorbent assay (ELISA) (R&D Systems, Inc., MN, USA) and the Bead CXCL13 assay (Mikrogen, Neuried, Germany) for the detection of CXCL13 in CSF.
View Article and Find Full Text PDFThe diagnosis of Lyme neuroborreliosis (LNB) is based on neurological symptoms, cerebrospinal fluid (CSF) pleocytosis, and intrathecally produced -specific antibodies. In most cases, the presence of intrathecally produced -specific antibodies is determined by using an enzyme-linked immunosorbent assay (ELISA). The edge effect is a known phenomenon in ELISAs and can negatively influence the assay reproducibility and repeatability, as well as index calculations of sample pairs which are tested in the same run.
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