Kinetic enzymatic determination of creatinine by a rapid semiautomated procedure.

The enzymatic method of creatinine determination, based on the transformation of creatinine to creatine by creatinine amido-hydrolase (EC 3.5.2.

Reference values for alanine and aspartate aminotransferase (ALT and AST) optimized by addition of pyridoxal phosphate.

The increases of the activity of aspartate and alanine transaminases observed in sera of healthy male and female subjects by addition of pyridoxal phosphate have been determined. The reference values of the activated reactions, distinguished by sex and age classes, are presented.