Publications by authors named "T Waku"
Reversibly photoswitchable chemical tools have aided in the development of novel approaches in the biomedical field. The visible region of light should be ideal for the biological application of this approach because of its low phototoxicity and deep penetration depth compared to ultraviolet light. Herein, we report a photoswitchable centromere-associated protein E (CENP-E) inhibitor, which is controllable with low-energy blue-green light (around 500 nm) illumination.
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- - A new group of inhibitors designed to target the mitotic motor protein CENP-E was developed using a chemical method that combines 1,3-diketones with different hydrazines under gentle conditions.
- - The research successfully explored how the structure of the pyrazole photoswitch affects the activity of these CENP-E inhibitors in lab tests (in vitro) and living cells.
- - This study enhances our understanding of how these inhibitors work and their potential applications in biological systems.
Benzo[a]pyrene-modified oligonucleotides were developed for the detection of RNAs with a point mutation. The probes produced two distinct fluorescence signals in response to single nucleotide differences in the RNA sequences, allowing for discrimination between the matched and single base mismatched RNA sequences in colorimetric and ratiometric manners.
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- Developed a CENP-E inhibitor that can be activated or deactivated using specific light (405 nm) and thermal relaxation.
- The inhibitor allows for precise control over chromosome movement in a single mitotic cell, using a photoswitching mechanism.
- This method successfully achieved targeted chromosome gain and loss in daughter cells by enabling asymmetric chromosome segregation.
An arylazopyrazole-based covalent inhibitor targeting the mitotic motor protein of centromere-associated protein E (CENP-E) was developed. Using this photoswitchable inhibitor, a photoswitchable CENP-E was chemically constructed in cells, which enabled to local control of mitotic cell division with light illumination.
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