Flow cytometry assay modifications: Recommendations for method validation based on CLSI H62 guidelines.

The Clinical and Laboratory Standards Institute (CLSI) H62-Validation of Assays Performed by Flow Cytometry guideline, released in 2021, provides recommendations for platform workflow and quality system essentials, instrument setup and standardization, assay development and optimization and fit-for-purpose analytical method validation. In addition, CLSI H62 includes some recommendations for the validation strategies after a validated flow cytometric method has been modified. This manuscript builds on those recommendations and discusses the impact of different types of assay modifications on assay performance.

Implementation of flow cytometry testing on rare matrix samples: Special considerations and best practices when the sample is unique or difficult to obtain.

The publication of Clinical and Laboratory Standards Institute's guideline H62 has provided the flow cytometry community with much-needed guidance on development and validation of flow cytometric assays (CLSI, 2021). It has also paved the way for additional exploration of certain topics requiring additional guidance. Flow cytometric analysis of rare matrices, or unique and/or less frequently encountered specimen types, is one such topic and is the focus of this manuscript.

An integrated methodology for assessing ecosystem response to environmental stressors under changing climatic conditions in coastal wetlands.

Three cores were taken along the salinity gradient (n-s) in the coastal wetlands of Louisiana; an intermediate marsh, a brackish marsh, and a mangrove swamp. The cores display remarkable stratigraphic and chronologic correlations, representing six successive ecosystems and environments, namely: interdistributary bay, freshwater marsh/swamp, deltaic lake, freshwater marsh/swamp, intermediate marsh, and brackish/saline. Sedimentary, geochemical, and palynological data were used to reconstruct the paleoenvironments, including ambient environment and ecosystem types.

The use of multivariate PCA dataset in identifying the underlying drivers of critical stressors, looking at global problems through a local lens.

Palynology-based multivariate datasets including geological, ecological, and geochemical data identified the relative importance of the underlying drivers of critical stressors to coastal wetlands by identifying and distinguishing between fluvial flooding, saline water intrusion, delta switching, and the landward migration of coastal plants. A sediment core was retrieved using a vibracorer from an intermediate marsh in Lake Salvador, Louisiana, USA. X-ray Fluorescence (XRF) quantified fluvial and marine elemental concentrations (Cl, Sr, Ca, Mn, K, Ti, Fe, Zn, Zr, Br).

Identifying forcing agents of environmental change and ecological response on the Mississippi River Delta, Southeastern Louisiana.

Freshwater wetlands on the Mississippi River delta plain, containing extensive swamps and marshes, have experienced land loss of 5197 km since the 1930s as the ocean has transgressed landward, resulting in landward retreat of bottomland forest, and transition of fresh to intermediate marsh. The timing and rapidity of these ecotonal transitions, and the impacts of natural and anthropogenic forces on this deltaic environment are uncertain. This study details a 448 cm sediment core retrieved from the intermediate marsh on the northern edge of Lake Salvador in southeastern Louisiana.