Perilymphatic fistula caused by Eustachian tube air inflation.

We present a case of a perilymphatic fistula (PLF) caused by Eustachian tube air inflation (ETAI) that was diagnosed using cochlin-tomoprotein (CTP) testing and successfully treated using transcanal endoscopic ear surgery to seal the inner ear window. A 77-year-old woman developed hearing loss and dizziness after undergoing ETAI at a local ear, nose, and throat clinic. Despite initial bed rest and steroid pulse therapy, the hearing did not improve, and transcanal endoscopic ear surgery was performed to repair the PLF.

Human enteric glia diversity in health and disease: new avenues for the treatment of Hirschsprung disease.

Background And Aims: The enteric nervous system (ENS), comprised of neurons and glia, regulates intestinal motility. Hirschsprung disease (HSCR) results from defects in ENS formation, yet while neuronal aspects have been extensively studied, enteric glia remain disregarded. This study aimed to explore enteric glia diversity in health and disease.

Investigation of Efficient Pullulan Synthesis Utilizing Huangjiu Lees as a Substrate.

Pullulan is a high-value biopolymer synthesized by through the fermentation of starch and sugars. It finds extensive applications in food, packaging, biomedicine, and other sectors. However, the high production costs significantly limit the development and application of pullulan.

Protocol for microindentation analysis of human cartilage and methacrylated gelatin hydrogels with varying stiffness in ATDC5 cell cultures.

Here, we present a protocol for evaluating mechanical properties of human cartilage specimens and methacrylated gelatin (GelMA) hydrogels with varying Young's moduli for cultures of ATDC5 chondrocytes using microindentation. We describe steps for preparing human cartilage specimens and hydrogels, culturing ATDC5 cells, and assessing the stiffness. This protocol was designed to facilitate reproducibility in biomechanical assays of chondrocytes.