Radioligand binding assay determination of epidermal growth factor receptor in ovarian tumours.

Purpose: To introduce a quantitative method for determination of epidermal growth factor receptor (EGFR) expression in tissue samples taken from normal ovaries, benign and malignant ovarian tumours, convenient for routine tests.

Materials And Methods: About 1g of tissue was taken intraoperatively from 136 patients; 105 of them had histologically verified ovarian tumours (64 malignant, 42 benign) and 30 had normal ovaries. The tissue was frozen, preserved and transported in liquid nitrogen (-196 degrees C).