[A modified method of deglycerinization of defrosted erythrocytes, cryopreserved in liquid nitrogen].

Specialists from Research Institute of Hematology and Transfusiology have developed a modified method of defrosted red cells deglycerinization which implies 2 cycles of centrifugation and using small quantities (625 ml) of low-cost and available washing solutions of sodium chloride. Simple performance and cost efficacy of this method make red cell cryopreservation more convenient for practice.

[Prolonged storage at +4 degrees C of cryopreserved and thawed erythrocytes].

A new method of taking out of storage is offered for red cells cryopreserved in the presence of low-concentration glycerin (15-20%) in relatively low temperature (-38 +/- 2 degrees C) and in liquid nitrogen. The new approach implies washing of erythrocytes not immediately after thawing but just before the transfusion. The delay in thawed erythrocytes washing warrants their safety and functional capacity throughout 3-week storage at + 4 degrees C after cryopreservation at -38 +/- 2 degrees C and within 2 weeks after freezing in liquid nitrogen.