PHARMACOGENETIC EFFECTS OF METHOTREXATE (MTX) IN UKRAINIAN PATIENTS DEPENDING ON THE MTHFR GENOTYPES (CLINICAL CASES).

The aim of the study is an evaluation the influence of MTHFR gene polymorphisms in the response to MTX in Ukrainian patients. The study group included unrelated Ukrainian patients with dermatoses - psoriasis vulgaris, psoriasis arthritis, psoriasis erythrodermic, atopic dermatitis, scleroderma, eczema, flat lichen and ectopic pregnancy. The materials for this study are based on the accumulated data from clinical observations of different reactions of patients with dermatoses and ectopic pregnancies when using methotrexate as the basic therapy according protocols.

[Biological role of fetuin A and its potential importance for prediction of cardiovascular risk in patients with type 2 diabetes mellitus].

The authors' data and those from literature concerning biological role of fetuin A glycoprotein have been generalized in the article. A direct correlation has been established between fetuin A and some adipokines involved in the formation of insulin resistance and atherogenesis (progranulin, omentin-1), and osteoprotegerin (the novel cardiovascular risk factor) as well as an increase of circulating levels of fetuin A in patients with type 2 diabetes mellitus with high cardiovascular risk metabolic pattern but without manifestations of macrovascular complications. This substantiates the involvement of fetuin A in the complex of biomarkers of subclinical atherosclerosis.

[Q192R polymorphism of PON-1 gene in type 2 diabetic patients].

Polymorphism of PON-1 gene in 192 position of amino acid sequence of enzyme paraoxonase 1 was studied. For this research we have used the blood samples of 96 patients with T2DM and 123 healthy habitants of Kharkiv. Frequencies of alleles for the patients (pQ = 0,65, pR = 0,35) and the healthies (PQ = 0,70 and pR = 0,30) did not differentiate meaningfully.

[Single nucleotide polymorphism of +276 g > t adiponectin (APM1) gene in donors of Kharkiv population].

Polymorphism of adiponectine (APM1) gene in +276G > T position was studied. For this research we used blood samples of 103 donors (men/women: 65/38; 70 Ukrainian, 33 Russian) - habitants of Kharkiv. Frequencies of T and G alleles were pT = 0.

[Polymorphism of paraoxonase (PON-1) gene in Slavonic part of Kharkiv population].

Polymorphism of PON-1 gene in 192 position of amino acid sequence of enzyme paraoxonase 1 related to the synthesis of allozymes R or Q was studied. For this research we used blood samples of 109 donors (men/women: 78/31; 84 Ukrainian, 25 Russian)--habitants of Kharkiv. Frequency of Q and R alleles were pQ = 0.