[Analysis of the cell tissue culture contamination with the bovine viral diarrhea virus and mycoplasmas].

Different cell tissue cultures and commercial fetal calf sera (FTS) used in biological and virological research were screened for the bovine viral diarrhea virus (BVDV, Pestivirus genus, Flaviviridae family) and mycoplasma contamination. BVDV was detected using RT-PCR and Indirect immunofluorescence (with monoclonal antibodies) methods in 33% cases of the studied cell lines and in > 60% cases of FCS. BVDV was shown to present and reproduce in high spectra of human cell lines, as well as in monkey, pig, rabbit, goat, dog, and cat cells at high levels (up to 100-1000 genome-equivalent copies per cell) and reached up to 10(3)-10(7) genome-equivalent copies per serum ml.

Contamination of cell cultures with bovine viral diarrhea virus (BVDV).

The incidence of contamination of cell strains used in biological and virological studies and of fetal calf sera (FCS) manufactured by Russian and foreign companies used for cell culturing with noncytocidal bovine viral diarrhea virus (BVDV; Pestivirus, Flaviviridae) was analyzed. The virus was detected by reverse transcription PCR and indirect immunofluorescence with monoclonal antibodies to BVDV virion envelope glycoprotein in 25% of 117 cell strains and 45% of 35 tested FCS lots. The virus multiplied and persisted in a wide spectrum of human cell strains and in monkey, swine, sheep, rabbit, dog, cat, and other animal cells.

[Effect of prolonged dietary zeolites on survival rate and intestinal reaction toward irradiation in mice of different age].

In model experiments on mice the influence of prolonged dietary consumption of zeolites on a survival rate, an average lifetime and a status of adherent mucous layer of a digestive tube was investigated. It was found that zeolite increased a survival rate and average lifetime in 55- and 95-day old mice exposed to a dose of 4 Gy. Zeolite shivirtuin caused more expressed effect, than pegasin.

[Forming of the Pavlov pouch changes functional condition of the adherent mucosal layer in upper regions of the digestive tract].

In result of forming the Pavlov small gaster, secretion of pepsine in isolated part and, probable, in other parts of the gaster, was increased. This entailed a decrease in the pepsin sorption level on the luminal surface of adherent mucous layer of the gaster parts and intestine under study. This led to a drop of the level of structure glycoproteins and to increase in the per cent contents of polymeric glycoproteins in the whole pool of the mucous layer glycoproteins.

[The condition of the intestinal mucosa of the proximal digestive tract subjected sectioning of the jejunal wall].

The balance between secretion and degradation of the mucosa glycoproteins in the dog intestine is preserved with the aid of local reflexes in both serous-muscular and mucous-submucous layers of the wall. Irrespective of major alterations in the glycoproteins synthesis in the mucosa, protective properties of the submucous layer are maintained owing to a high content of acetylneuraminic acid and low level of mucous glycoproteins degradation.