Developing Topics.

Background: Mitochondrial DNA copy number (mtDNAcn) quantifies the number of mitochondria genomes per nucleated cell, with reduced mtDNAcn being associated with increased Alzheimer's disease (AD) neuropathology. Blood-based mtDNAcn has technical confounders, such as DNA purification, and biological confounders, such as compensatory upregulation of mtDNA. Therefore, we optimized a protocol for mtDNAcn quantification using droplet digital PCR (ddPCR) by testing (i) whole peripheral blood mononuclear cells (PBMCs) vs platelet-depleted PBMCs, (ii) column-based DNA extraction vs cell lysate, and (iii) mitochondrial DNA replication (mtDNArep).

Developing Topics.

Background: Different pathologies can cause dementia, including Alzheimer's disease (AD), dementia with Lewy bodies (DLB) and frontotemporal dementia. Understanding the biological mechanisms underlying these diseases is important in order to develop therapies. Here we performed cerebrospinal fluid (CSF) proteomics in AD, DLB and FTD in order to study proteomic changes and identify novel potential biomarkers.

Cardiopulmonary Exercise Test Interpretation Across the Lifespan in Congenital Heart Disease: A Scientific Statement From the American Heart Association.

Survivorship from congenital heart disease has improved rapidly secondary to advances in surgical and medical management. Because these patients are living longer, treatment and disease surveillance targets have shifted toward enhancing quality of life and functional status. Cardiopulmonary exercise testing is a valuable tool for assessing functional capacity, evaluating cardiac and pulmonary pathology, and providing guidance on prognosis and interventional recommendations.

Glycine Decarboxylase Regulates Renal Carcinoma Progression via Interferon Stimulated Gene Factor 3-Mediated Pathway.

Renal cell carcinoma (RCC) is considered as a "metabolic disease" due to various perturbations in metabolic pathways that could drive cancer development. Glycine decarboxylase (GLDC) is a mitochondrial enzyme that takes part in the oxidation of glycine to support nucleotide biosynthesis via transfer of one-carbon units. Herein, we aimed to investigate the potential role of GLDC in RCC development.

First formal record of the feeding habits of Saileriolidae (Hemiptera, Heteroptera, Pentatomomorpha, Pentatomoidea), with redescription of (Schaefer & Ashlock, 1970), comb. nov. endemic to Vietnam.

In the present study, the rare true bug (Schaefer & Ashlock, 1970), (Hemiptera, Heteroptera, Pentatomomorpha, Pentatomoidea, Saileriolidae), which is endemic to Vietnam, is redescribed and transferred from the genus China & Slater, 1956 to the genus Hsiao, 1964 based on morphological characteristics. Adults and nymphs of this species congregate in groups of several individuals and suck sap from the abaxial side of the leaves of sp. (Lauraceae).