Publications by authors named "T V Pestova"
Polypeptides arising from interrupted translation undergo proteasomal degradation by the ribosome-associated quality control (RQC) pathway. The ASC-1 complex splits stalled ribosomes into 40S subunits and nascent chain-tRNA-associated 60S subunits (60S RNCs). 60S RNCs associate with NEMF that promotes recruitment of the RING-type E3 ubiquitin (Ub) ligase Listerin (Ltn1 in yeast), which ubiquitinates nascent chains.
View Article and Find Full Text PDFExamination of all publicly available Oryctolagus cuniculus (rabbit) ribosome cryo-EM structures reveals numerous confusing inconsistencies. First, there are a plethora of single nucleotide differences among the various rabbit 28S and 18S rRNA structures. Second, two nucleotides are absent from the NCBI Reference Sequence for the 18S rRNA gene.
View Article and Find Full Text PDFArticle Synopsis
- * Researchers discovered multiple avian calicivirus genomes featuring type 2 IRESs and identified an additional open reading frame (ORF1*) that overlaps with the main ORF1, suggesting complex genomic features in these viruses.
- * The study confirmed that ribosomes can initiate translation at multiple sites on the avian calicivirus genomes, and this process requires specific initiation factors, with enhanced initiation occurring thanks to IRES-binding factors that interact with key
Article Synopsis
- Viral internal ribosomal entry sites (IRESs) are RNA elements that enable translation to start without the usual requirement for a cap structure, and they come in different classes based on their structural features.
- Research has uncovered new types of IRESs that vary in size and complexity, with some having tandem pseudoknots and others showing unique structural elements that enhance their function.
- The study also suggests that IRESs evolve through mechanisms such as the incremental addition of structural components and recombination of different RNA sequences, helping to explain their diversity across various viral genomes.
Ribosomal stalling induces the ribosome-associated quality control (RQC) pathway targeting aberrant polypeptides. RQC is initiated by K63-polyubiquitination of ribosomal protein uS10 located at the mRNA entrance of stalled ribosomes by the E3 ubiquitin ligase ZNF598 (Hel2 in yeast). Ubiquitinated ribosomes are dissociated by the ASC-1 complex (ASCC) (RQC-Trigger (RQT) complex in yeast).
View Article and Find Full Text PDF