[The adaptive mechanisms of cardio- and angioprotective action of reflexotherapy].

The adaptive mechanisms of action of reflexotherapy are analysed. It was shown in physiological and biochemical experiments on the whole organism, the isolated hearts and strips of resistive arteries of animals that stimulation of acupuncture points by different physical factors increased the activity of opioid, antioxidative, and other stress-limiting systems, reduced secretion of corticosteron during stress, stimulated biosynthesis of stress proteins, induced cyto- and cardioprotective effects, prevented or limited functional and structural hyperadrenal damages. Clinical investigations demonstrated significant hypothensive, anti-ishemic and anti-arrhythmic effects of reflexotherapy, improved resistance of healthy subjects and patients with cardiovascular diseases to physical and emotional loadings.

[Assessment of bronchopulmonary immunity status for Mayak PA workers at the late period after prolonged exposure].

Samples of induced sputum from 187 individuals were used to estimate the bronchopulmonary immunity status after a prolonged radiation exposure (120 individuals who are the Mayak main plant workers were exposed to combined internal a-radiation due to incorporated plutonium (239Pu) and external y-radiation during their career). The control group included 67 Ozersk residents of a corresponding age and gender, without any occupational exposure, who were examined at the same period. The immune system is the most important component in homeostasis.

[The correction of cognitive and psychological disorders in the patients presenting with vertebrogenic pain syndrome].

The objective of the present study was the dynamic estimation of the intensity of pain syndrome with the use of a visual-analog scale (VAS), characteristics of the psychological status and cognitive disorders, quality of life, and brain electrogenesis in 50 patients presenting with vertebrogenic pain syndrome of lumbar-sacral localization. It was shown that long-standing pain syndrome plays the dysadaptive role, causes structural and functional changes in the central nervous system (CNS), and is responsible for psychoemotional and cognitive disorders. The introduction of transcranial stimulation in the combined treatment of such patients promotes correction and reversibility of the above disorders, enhances the efficacy of electrotherapy, and improves the quality of life in the subjects with vertebrogenic pain syndrome.

[The application of trans-auricular low-frequency electrical puncture for the treatment of arterial hypertension].

Clinical studies involving 67 patients with arterial hypertension (AH) have demonstrated that a single course of trans-auricular low-frequency electrical puncture produced a marked stress-relieving effect that was manifested as the decreased anxiety level, normalization of neurohumoral regulation of arterial pressure and central hemodynamics, improved tolerance of controlled psycho-emotional loading, beneficial effect on cerebral blood circulation, lipid and electrolyte metabolism. In addition, the proposed therapeutic modality resulted in substantial reduction of arterial pressure. Taken together, these observations give reason to consider it as a promising tool for the treatment of patients presenting with arterial hypertension.

[The synthesis and mass spectrometry of oligonucleotides bearing thiophosphoryl modifications of the predetermined localization].

The technique of parallel automated synthesis of oligodeoxynucleotides bearing various local thiophosphoryl internucleotide bonds was optimized using assembling in the standby mode and creation of special program blocks. The selected conditions of Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI TOF MS) provided an increase in the method sensitivity (up to 1-10 fmol of oligonucleotide in sample) and registration of reliable spectra of oligodeoxynucleotide thiophosphoryl analogues. This enables to reliably prove the presence of the specified number of thiophosphoryl bonds within synthetic sequences.