Kidney and liver function in rats during the edema following constriction of thoracic inferior vena cava with and without adrenalectomy or hypophysectomy.

In several animal species, constriction of the thoracic inferior vena cava (TIVCC) is known to increase proximal sodium reabsorption and inhibit natriuresis following saline loading, leading to edema. To eluicidate the role of adrenal and hypophyseal hormones in the development of edema, kidney and liver functions after TIVCC were compared in adrenalectomised or hypophysectomised rats, and in intact controls. It was found that edema (body weight increase) and kidney and liver affliction were much less pronounced after the operations.

[Use of albuminous standards in the quantitative cytochemistry of dehydrogenases].

A method for preparing albuminous standards with various concentrations of NBT formazans is proposed. Using these standards, the molar extinction coefficients for half and fully reduced formazans of NBT in the region from 450 to 650 nm were calculated. Since the absorption curves of half and fully reduced NBT formazans are identical either in vitro in the standards or enzymatically in tissues, the extinction coefficients calculated on the basis of these standards may be used for densitometrical estimation of cellular dehydrogenase activity.

[Succinate dehydrogenase and Na+-K+-ATPase activities in cells of rat kidney tubules during increased excretion of sodium, caused by furosemide].

In the cells of nephron tubules an effect of furosemide on kidney function and on succinate dehydrogenase and Na+-K+-ATPase activities was studied cytochemically. Administration of furosemide (1 mg per 100 g of body weight) increased excretion of sodium and potassium. If the rate of filtration through Malpighian tufts was constant, the increase in Na and K excretion demonstrated the tubular effect of the diuretics.