[Cryopreservation of erythrocytes at moderately low temperature with transportation simulation].

Cryopreservation of red cell mass at -75-80 degrees C as concentrates warrants its prolonged storage (up to 1.5 years) in functional condition and transfusion without centrifugation. The above cryoconcentrates are resistant to temperature fluctuations and keep morphologically and functionally safe in response to a temperature rise to -32 degrees C (transportation simulation).

[Effect of temperature, daunorubicin concentration and suspension hematocrit on daunorubicin binding by human erythrocytes].

When incubated in the isotonic medium containing daunorubicin, human erythrocytes bound daunorubicin. At the initial concentration of daunorubicin in the medium of not higher than 1 mg/ml, the suspension hematocrit of about 30 per cent and the temperature of 25 degrees C the maximum binding of daunorubicin was achieved in approximately 30 minutes and was equal to about 80 per cent of the initial content. When the temperature was lowered from 30 to 15 degrees C the initial rate of the daunorubicin binding by the erythrocytes decreased several times.

[Nephrogenic mechanism of post-traumatic hemolysis].

The crush-syndrome model was introduced for experimental evaluation of the kidney-related mechanism of red cell destruction in an early posttraumatic period (1 day). Absolute and relative counts of the red cells, HCT were found reduced, while serum hemoglobin was on the increase in rats with crush syndrome against the controls and rats subjected to trauma and nephrectomy. Renal perfusion in situ with syngeneic blood brought about a decline in rat erythrocyte functional performance and a rise in the level of free hemoglobin.